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| 重组人肝增强因子工程菌JM109的发酵工艺研究 | |
| 引用本文： | 李茹冰,单中元,付泳航,杨联萍,易学瑞,孔祥平. 重组人肝增强因子工程菌JM109的发酵工艺研究[J]. 中国生化药物杂志, 2000, 21(2): 63-65 |
| 作者姓名： | 李茹冰 单中元 付泳航 杨联萍 易学瑞 孔祥平 |
| 作者单位： | 1. 510602,广州,中国人民解放军传染病中心分子生物学科 2. 通辽市哲里木盟传染病医院 3. 空军第458医院检验科 |
| 摘 要： | 目的：研究表达重组人肝增强团子（hALR）工程菌JM109的发酵工艺。方法：采用发酵罐发酵，优化工程菌发酵的培养基配方、pH值、诱导表达时间、补料方式等。结果：在pH6．9条件下，培养6h后诱导表达5h，同时补科，5L罐发酵工程菌，菌体收得量可达湿重33．0g／L。目标蛋白表达量约占菌体总蛋白的31．3％。结论：此发酵工艺可以较好地提高ALR工程菌菌体得率和目标蛋白表达量。 |
| 关 键 词： | 人肝增强因子 发酵 大肠杆菌 |
| 修稿时间： | 1999-07-28 |
Study on the Fermentation Process of E. cloi JM109 Expressing Human Augmenter of Liver Regenration |
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| Li Rubing,Shan Zhongyuan,Fu Yonghang,Yang Lianping,Yi Xuerui,Kong Xiangping. Study on the Fermentation Process of E. cloi JM109 Expressing Human Augmenter of Liver Regenration[J]. Chinese Journal of Biochemical Pharmaceutics, 2000, 21(2): 63-65 | |
| Authors: | Li Rubing Shan Zhongyuan Fu Yonghang Yang Lianping Yi Xuerui Kong Xiangping |
| Affiliation: | Li Rubing,Shan Zhongyuan,Fu Yonghang,Yang Lianping,Yi Xuerui,Kong Xiangping(Department of Molecular Biology, Infectious Disease Center, the 458thHospital of PLA Guangzhou 510602) |
| Abstract: | Purpose : To develop the best fermentation process on E. coli JM109 expressing human augmenter of liver regenration (hALR ). Methods: Optimizing the composition of the medium,the range of pH, induction time and the way of fed-batch in fermentation tank. The expression of recombinant protein was analyzed. Stable fermentation parameters were obtained. Results:Under the established conditions,33. 0g of wet bacteria per liter could be obtained,and the derivative of recombinant hALR was about 31. 3% of total protein in the host, Conclusion: The developed fermentative technology might increase the collection ratio and expression of recombinant hALR. |
| Keywords: | Recombinant hALR Fermentation E. coli |
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