Dynamic changes of glycine and taurine in corpus striatum of rats with ischemia-reperfusion injury and effects of electroacupuncture |
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| Authors: | Ming-shu Xu Lin-Bao Ge Xin-hui Wang Jia Xu Zhi-qiang Wang Yi-jun Cui |
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| Institution: | (1) Shanghai Research Institute of Acupuncture and Meridian, Shanghai, 200030, P. R. China;(2) Shanghai Research Center for Acupuncture and Meridian, Shanghai, 201203, P. R. China;(3) Shanghai Research Institute of Qigong, Shanghai, 200030, P. R. China;(4) Yueyang Hospital of Integrated Chinese and Western Medicine affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200047, P. R. China |
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| Abstract: | Objective To investigate the dynamic changes of glycine (Gly) and taurine (Tau) in corpus striatum of rats with ischemia-reperfusion
injury (IRI), and the regulation effect of electroacupuncture on them.
Methods Thirty SD rats were randomly divided into sham surgery group, model group, and treatment group. Rats in the sham surgery group
were operated without ischemia. Rats in the model group and treatment group were operated to make model of cerebral IRI. The
ischemia status lasts 1.5 h, and reperfusion was performed for 3.75 h. Rats in the treatment group were treated with electroacupuncture
at Fengchi (GB 20). Neurologic deficit score (NDS) was used to evaluate the rats functions. Microdialysis and High Performance
Liquid Chromatography technique were used to detect the changes of Gly and Tau in corpus striatum of rats.
Results Contents of Gly and Tau increased at 1.5 h after ischemia. Them decreased to level as same as those rats in the sham surgery
group after reperfusion. The content of Gly in the model and treatment group increased again at 2–2.5 h after reperfusion,
and then decreased to the same level of the sham surgery group. There was a third increase of Gly content in the treatment
group at 2.75 h after reperfusion. Electroacupuncture treatment could delay the decrease of Tau content after reperfusion,
and make it increase at 1.5, 2 and 2.75 h after reperfusion. The peak appeared in the last time. NDS in the model and treatment
group were more than that in the sham surgery group, and lowered at 5.25 h after surgery. Effects in treatment group was better
than that in the model group (P<0.05).
Conclusion IRI makes contents of Gly and Tau in corpus striatum increase at a special time. Electroacupuncture treatment could increase
contents of Gly and Tau at a special time after reperfusion, which maybe an important mechanism of protecting effects of electroacupuncture
on the brain.
GE Lin-bao (1951–), male, research fellow |
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| Keywords: | Microdialysis Brain Ischemia Electroacupuncture Reperfusion Injury Glycine Taurine Rats |
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