缺氧导致体外培养乳鼠心肌细胞凋亡的研究

目的:通过体外培养乳鼠心肌细胞模拟缺氧模型,探讨心肌细胞缺氧损伤时是否发生心肌细胞凋亡。方法:取(1~3)d新生SD乳鼠心肌进行心肌细胞培养,采用模拟缺氧模型,通过HE染色、流式细胞仪等手段观察检测细胞凋亡的改变。结果:在本实验心肌细胞缺氧模型中,HE染色可见凋亡细胞体积变小,胞核浓缩,胞质嗜酸性增强。流式细胞仪检测出明显的凋亡峰。结论:本实验培养乳鼠心肌细胞缺氧模型可导致心肌细胞凋亡,可通过HE染色观察凋亡细胞形态学的变化,流式细胞术检测细胞DNA含量而对凋亡细胞进行定量分析。

Study on the Effect of Hypoxia on Apoptosis of Cultured Newly Born Rat Cardiac Myocytes

Objective To investigate the possible hypoxia-mediated cellular apoptosis after ischemic cardiac injury via a model of cultured newly born rat cardiac myocytes. Methods Cardiac myocytes cultures from newly born rats (1~3d) were examined for apoptosis with HE stain and flow cytometry after cultured 96h and again examined after exposure to hypoxic enviroment for 16h. Results Apoptotic changes were evident in the hypoxic culture cells. The HE stain revealed cellular shrinkage, nuclear chromosomal condensation with cytoplasmic eosinophilism. Also, distinct aopptosis peak was observed in the flow cytometry. Conclusion This experiment proved that hypoxic model of cardiac myocyte culture showed definite apoptosis of the cells.