| Nanog表达上调促进乳腺癌细胞MCF-7的增殖和侵袭 |
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| 引用本文： | 孙秀梅,张飞,田然,刘媛,王智勇,杨毅,魏熙胤,牛瑞芳.Nanog表达上调促进乳腺癌细胞MCF-7的增殖和侵袭[J].天津医科大学学报,2014,20(6):421-425. |
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| 作者姓名： | 孙秀梅 张飞 田然 刘媛 王智勇 杨毅 魏熙胤 牛瑞芳 |
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| 作者单位： | 天津医科大学肿瘤医院公共实验室,国家肿瘤临床医学研究中心,天津市“肿瘤防治”重点实验室,乳腺癌防治教育部重点实验室,天津300060 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的：观察Nanog高表达对乳腺癌细胞MCF-7生物学特性的影响。方法：构建重组质粒pcDNA3.1（-）-Nanog,利用免疫荧光观察Nanog高表达后,乳腺癌细胞MCF-7中Nanog的定位。利用平皿克隆分离法获得Nanog高表达的乳腺癌细胞系MCF-7。应用克隆形成实验、Transwell迁移和侵袭实验观察Nanog高表达对乳腺癌细胞MCF-7生物学特性的影响。结果：Nanog在MCF-7细胞中定位于核,Nanog高表达后,乳腺癌细胞MCF-7的增殖、迁移和侵袭能力显著增强。结论：Nanog能够促进乳腺癌细胞MCF-7的增殖、迁移和侵袭能力。
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| 关 键 词： | Nanog 增殖 迁移 侵袭 MCF-7 乳腺癌 |
Up-regulation of Nanog promotes cell proliferation and invasion in breast cancer cells MCF-7 |
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| SUN Xiu-mei,ZHANG Fei,TIAN Ran,LIU Yuan,WANG Zhi-yong,YANG Yi,WEI Xi-yin,NIU Rui-fang.Up-regulation of Nanog promotes cell proliferation and invasion in breast cancer cells MCF-7[J].Journal of Tianjin Medical University,2014,20(6):421-425. |
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| Authors: | SUN Xiu-mei ZHANG Fei TIAN Ran LIU Yuan WANG Zhi-yong YANG Yi WEI Xi-yin NIU Rui-fang |
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| Institution: | (Department of Public Laboratory, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Can- cer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin 300060,China) |
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| Abstract: | Objective： To observe the effects of up-regulation of Nanog on the biological characteristics of breast cancer cells MCF-7.Methods： A recombinant plasmid pc DNA3.1（-）-Nanog was constructed. Using immunofluorescence, Nanog positioning in MCF-7 was observed after transfected with plasmid pc DNA3.1（-）-Nanog. Breast cancer cell line MCF-7 high expression Nanog was obtained by using cloning separation plate. The effects of up-regulation of Nanog on the biological characteristics of MCF-7 breast cancer cells were observed by clonogenic assay, Transwell migration and invasion assay. Results： Nanog was localized in the nucleus in MCF-7 cells. MCF-7 high expression of Nanog had more significant effect on cell proliferation, migration and invasion. Conclusion： Up-regulation of Nanog could promote cell proliferation, migration and invasion of MCF-7. |
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| Keywords: | Nanog proliferation migration invasion MCF-7 breast cancer |
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