静脉麻醉药对脂多糖诱导的神经星形胶质细胞增殖的影响及其机制

目的 检测静脉麻醉药对脂多糖诱导的神经星形胶质细胞的影响及其机制。 方法 利用MTT实验、集落形成实验和Annexin V-FITC/PI双染色法检测静脉麻醉药对脂多糖作用后的神经星形胶质细胞增殖和凋亡的影响。Western blotting技术检测相关机制。 结果 静脉麻醉药可以逆转脂多糖诱导的神经星形胶质细胞增殖。经麻醉药处理后的神经星形胶质细胞p-Akt和p-mTOR蛋白表达下降，Caspase-3和Caspase-9表达水平升高。结论 静脉麻醉药可以通过抑制mTOR通路逆转脂多糖诱导的神经星形胶质细胞增殖。

Effects of intravenous anesthetics on lipopolysaccharide-induced proliferation of glial cells and its mechanisms

Objective To detect the effect and mechanism of intravenous anesthetics on lipopolysaccharide (LPS) treated glial cells. Methods The proliferative ratio and the apoptotic ratio of glial cells after treated with LPS were detected by MTT assay, colony formation assay, and Annexin V-FITC/PI double staining, respectively. The protein related to the mechanisms was analyzed by Western blotting. Results Intravenous anesthetics reversed the LPS-induced proliferation of glial cells. The levels of p-Akt and p-mTOR were downregulated, while the levels of Caspase-3 and Caspase-9 were upregulated in intravenous anesthetics treated cells than untreated ones. Conclusion Intravenous anesthetics may reverse LPS-induced proliferation of glial cells by suppressing mTOR signaling pathway.