响应面分析法优化牛蒡根多糖提取工艺

目的:优化牛蒡根多糖的提取工艺.方法:采用水提醇沉法提取多糖,以多糖得率为考察指标；采用响应面分析法研究影响牛蒡根多糖测定的因素,以多糖提取率为响应值作响应面和等高线.结果:牛蒡多糖提取工艺的最佳条件为料液比1∶14.43,浸提温度84.85℃,浸提时间3.81 h.此条件下,牛蒡多糖的提取率为6.16％.结论:该优选工艺稳定、可行.

Optimization of Extraction Technology for Polysaccharide from Roots of Arctium lappa by Response Surface Methodology

Objective:To optimize extraction technology of polysaccharide from roots of Arctium lappa.Method: Polysaccharide was extracted by water extraction and alcohol precipitation method with yield of polysaccharide as index;Response surface methodology was used to study on influencing factors of determination of polysaccharide from roots of A.lappa,response surface and contour were made with extract ratio of polysaccharide as response value.Result: Optimum extraction technology conditions were solid-liquid ratio of 1∶14.43,extraction temperature 84.85 ℃,extracting time 3.81 h.Under these conditions,yield of polysaccharide from roots of A.lappa was 6.16%.Conclusion: This optimized technology was stable and feasible.