| 正交试验优选蜂病康合剂壳聚糖纯化工艺 |
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| 引用本文： | 贾永艳,祝侠丽,陈波,田效志,苗明三,樊静静.正交试验优选蜂病康合剂壳聚糖纯化工艺[J].中国实验方剂学杂志,2012,18(11):32-34. |
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| 作者姓名： | 贾永艳 祝侠丽 陈波 田效志 苗明三 樊静静 |
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| 作者单位： | 河南中医学院,郑州,450008 |
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| 基金项目： | 河南省重点科技攻关计划项目(072102130006) |
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| 摘 要： | 目的:探讨壳聚糖澄清剂用于蜂病康提取液的纯化工艺.方法:以纯化后药液中黄芩苷含量为指标,采用正交试验优选絮凝澄清条件,并与传统醇沉工艺比较.结果:正交优选工艺优于醇沉工艺,最佳絮凝澄清条件为水提液浓缩到1∶8,壳聚糖用量15％,温度50℃.结论:壳聚糖澄清剂可用于蜂病康合剂的精制.
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| 关 键 词： | 壳聚糖 正交实验 蜂病康合剂 纯化工艺 |
| 收稿时间： | 2011/12/12 0:00:00 |
Optimization of Purification Process for Chitosan from Fengbingkang Mixture by Orthogonal Test |
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| JIA Yong-yan,ZHU Xia-li,CHEN Bo,TIAN Xiao-zhi,MIAO Ming-san and FAN Jing-jing.Optimization of Purification Process for Chitosan from Fengbingkang Mixture by Orthogonal Test[J].China Journal of Experimental Traditional Medical Formulae,2012,18(11):32-34. |
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| Authors: | JIA Yong-yan ZHU Xia-li CHEN Bo TIAN Xiao-zhi MIAO Ming-san and FAN Jing-jing |
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| Institution: | Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China |
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| Abstract: | Objective:To investigate purification technology of Fengbingkang mixture with chitosan as clarification agent.Method: With the content of baicalin from purified liquid as index,flocculation clarification process was optimized by orthogonal design,and compared with traditional ethanol precipitation process.Result: These test result showed that optimized technology was better than ethanol precipitation technology.Optimum flocculation clarification process conditions were:water extraction liquid concentrated to 1∶8,the amount of chitosan 15%,temperature 50 ℃.Conclusion: Chitosan clarifying agent could be used for purifying Fengbingkang mixture. |
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| Keywords: | chitosan orthogonal test Fengbingkang mixture purification technology |
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