瘤内微淋巴管与喉鳞癌颈淋巴结转移的相关性研究

目的通过确定喉鳞癌组织内微淋巴管增殖情况及其与颈淋巴结转移之间的关系,为喉鳞癌转移和预后的判定以及治疗提供一定的理论依据。方法通过双重免疫病理学方法检测淋巴内皮透明质酸受体(LYVE)-1和Ki67在喉癌组织和淋巴管内皮的表达,并计数喉鳞癌组织中淋巴管密度,用统计学方法对淋巴管密度(LVD)与喉鳞癌各种临床病理参数进行分析,得出相关性结论。结果喉癌瘤体内存在LYVE-1(+)和Ki67(+)的淋巴管,LYVE-1和Ki67在喉癌组织和癌旁正常组织之间表达差异有统计学意义(P<0.01),LVD与N分期则存在相关性(P<0.05)。结论喉鳞癌瘤组织中存在新生微淋巴管;喉癌组织内淋巴管密度与喉鳞癌的淋巴转移之间存在相关关系。

Correlation between intratumoral microlymphatics and neck lymphatic metastasis of laryngeal squamous cell carcinoma

Objective To elucidate the relationship between microlymphatic proliferation and neck lymphatic metastasis of laryngeal squamous cell carcinoma （LSCC）, and to provide evidence for prediction of metastasis and prognosis in LSCC. Methods Double-staining immunohistochemical test was performed in 50 specimens of LSCC to determine the expression of LYVE-1 and Ki67. The lymphatic vessel density （LVD） was calculated. The correlation between LVD and clinical parameters of LSCC was analyzed statistically. Results LYVE-1 （＋） and Ki67（＋） microlymphatics were observed in LSCC. The expressions of LYVE-1 and Ki67 in LSCC tissues were significantly different from those in adjacent normal tissue （P〈0.01）. LVD was found to be associated with N staging. Conclusion New growth of microlymphatics was detected in LSCC tissues. There was a correlation between high levels of LVD and lymphatic metastasis of LSCC.