| 人尿中脱氢睾酮代谢物的GC/MS分析 |
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| 引用本文： | 张霁,刘春胜,周同惠. 人尿中脱氢睾酮代谢物的GC/MS分析[J]. 药学学报, 1991, 26(5): 362-366 |
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| 作者姓名： | 张霁 刘春胜 周同惠 |
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| 作者单位： | 中国医学科学院药物研究所分析室，中国医学科学院药物研究所分析室，中国医学科学院药物研究所分析室 北京 100050，北京 100050，北京 100050 |
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| 摘 要： | 本文用GC/MS方法,对人尿中脱氢睾酮(boldenone)代谢物进行了研究。志愿者口服20 mg脱氢睾酮后,收集阳性尿。尿样经过包括XAD-2树脂柱吸附、酶水解、有机相萃取及三甲基硅烷衍生化反应的预处理后,用毛细管气相色谱与质谱检测器联用进行分析,鉴定了脱氢睾酮的几个主要代谢物和它们的代谢模式;总结了兴奋剂检测中具有意义的代谢物及其碎片离子;对收集的阳性尿中脱氢睾酮浓度进行测定;对预处理方法的回收率进行了研究。
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| 关 键 词： | 脱氢睾酮 尿样分析 气相色谱质谱法 |
| 收稿时间： | 1990-03-03 |
GAS CHROMATOGRAPHIC/MASS SPECTROMETRIC ANALYSIS OF BOLDENONE URINARY METABOLITES IN MAN |
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| J Zhang,CS Liu and TH Zhou. GAS CHROMATOGRAPHIC/MASS SPECTROMETRIC ANALYSIS OF BOLDENONE URINARY METABOLITES IN MAN[J]. Acta pharmaceutica Sinica, 1991, 26(5): 362-366 |
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| Authors: | J Zhang CS Liu TH Zhou |
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| Affiliation: | Department of Analytical Chemistry, Chinese Academy of Medical Sciences, Beijing. |
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| Abstract: | The metabolism of boldenone (17 beta-hydroxy-1,4-androstem-3-one) in man has been investigated by gas chromatography/mass spectrometry. After oral administration of a 20 mg dose to man, six metabolites were detected in the conjugated fraction of the urinary samples. Boldenone, the major compound excreted in urine, was detected within 34 h after administration. In addition, several metabolites, resulting from the hydroxylation of boldenone and the reduction of the unsaturated carbon bonds of boldenone, were detected in the urine samples varying from 9 to 83 h. Extraction and fractionation of these metabolites were achieved by using XAD-2 column and gas chromatography. The recovery of the whole procedure was studied. Furthermore, the mass spectra of the metabolites are presented and major fragment pathways are discussed. |
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| Keywords: | Boldenone Urine analysis GC/MS |
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