乳腺癌患者血浆基质金属蛋白酶-2,-9的明胶酶谱分析

目的:探测乳腺癌患者血浆基质金属蛋白酶-2,-9(MMP-2,-9)活性水平与乳腺癌临床病理参量的可能关系.方法:利用光密度酶谱方法测定了20例健康人血浆和58例乳腺癌患者血浆的MMP-2,-9的活性水平.结果:乳腺癌患者血浆的MMP-2,-9活性水平均较对照组显著升高(P<0.01),两种酶活性水平的升高均与淋巴结转移和c-erbB-2的过量表达显著相关(P<0.05),两种酶的活性和雌激素受体水平呈负相关(P<0.05).结论:MMP-2,-9可能在乳腺癌的诊断和治疗上有着重要作用,有可能作为推测乳腺癌的侵袭,转移和预后的有用的分子.

Zymographic analyses of matrix metalloproteinase-2,-9 in plasmas of patients with breast cancer

AIM: To investigate the possible relationship between the activity levels of MMP-2,-9 in the plasmas of patients with breast caner and the clincopathological parameters. METHODS: The values of MMP-9 and MMP-2 in 20 healthy plasma samples and 58 cancer plasmas were determined by densitometric scan of zymograms. RESULTS: The activity levels of both MMP-2 and MMP-9 in breast cancer patients were significantly higher than those in control (P<0.01). The elevated activity levels of the enzymes were correlated with lymph node metastasis and c-erbB-2 overexpression (P<0.05). An inverse correlation was found between the enzymes and oestrogen receptor (ER) levels (P<0.05). CONCLUSION: MMP-2, -9 in the plasmas may play an important role in the diagnosis and treatment of breast cancer and they may serve as useful molecules in predicting tumor invasion, metastasis and prognosis.