骨硬化蛋白单克隆抗体对于有去卵巢大鼠骨折愈合影响的实验研究

目的探索骨硬化蛋白单克隆抗体(Scl-Ab)对去势大鼠股骨干骨折愈合影响的实验研究。方法 30只健康雌性SD大鼠随机行假手术(Sham,n=5)和切除双侧卵巢(OVX,n=25)手术12w后每组各取5只大鼠处死取股骨行双能X线仪检测确保骨质疏松的建立,随后OVX组大鼠建立左侧股骨干中段骨折模型随机的分成2组:OVX组及骨硬化蛋白单克隆抗体治疗(Scl-Ab)组。术后第1天Scl-Ab组开始给予药物治疗:Scl-Ab皮下注射(剂量25 mg/kg,每周2次)直至8 w,8 w时所有大鼠处死取股骨行Micro-CT、硬组织切片检测及HE组织切片检测。结果 Micro-CT结果显示:Scl-Ab组和OVX组相比,骨折端有较多的新生骨形成,且有较高BV/TV、Tb.Th、Tb.N和较低的Tb.Sp值,比较有明显的统计学意义。HE结果表明Scl-Ab组骨折端有更多的骨痂形成,且骨痂更加成熟。硬组织切片观察结果表明:Scl-Ab组骨折端骨痂矿化速度更快,和OVX组表明有统计学意义。结论 Scl-Ab通过增加骨折端骨量及促进骨组织矿化来治疗去卵巢大鼠股骨中段骨折的愈合。

The experimental study of effect of sclerostin antibody on fracture healing in ovariectomized rats

Objective This study was designed to investigate the effect of sclerostin-antibody (Scl-Ab) on the healing of femoral shaft fracture in OVX rats. Methods Thirty healthy female SD rats were randomly performed bilateral ovariectomy (n=25) or sham operation (n=5). In 12 weeks, 5 rats from each group were sacrificed. The femurs of the rats were collected for BMD evaluation to confirm the establishment of osteoporotic animal model. The left femoral shaft closed fracture model was created in all OVX rats. The rats were randomly divided into 2 groups: OVX group and Scl-Ab group. Rats in Scl-Ab group received 25 mg/kg of Scl-Ab, 2 times a week, for 8 weeks. The femurs of the rats were harvested and were evaluated with micro-computerized tomography. The undecalcified slices were observed with HE staining. Results Comparison with OVX group, more new bone formed in the fracture region in Scl-Ab group. BV/TV, Tb.Th, and Tb.N were higher, but Tb.Sp was lower in Scl-Ab group than those in OVX group. The results of HE staining showed that more callus formed and the callus was mature in Scl-Ab group. The observation of the undecalcified slices showed that the mineralization of the callus was faster in Scl-Ab group than in OVX group. Conclusion Scl-Ab can heal femoral shaft closed fractures by increasing bone mass and mineralization.