柴胡皂苷D对人肝癌HepG2细胞系增殖和裸鼠肝癌形成的抑制作用

目的：探索柴胡皂苷D(Saikosaponin-d,SSd)对人肝癌HepG2细胞系增殖和裸鼠肝癌形成的影响。方法：CCK-8检测细胞增殖；流式细胞术分析细胞凋亡；蛋白印记检测细胞Ki67和cleaved caspase-3表达；脱氧核糖核苷酸末端转移酶介导的缺口末端标记(TUNEL)染色分析肿瘤组织细胞凋亡；免疫组化检测肿瘤组织Ki67和cleaved caspase-3表达。结果：柴胡皂苷D组HepG2细胞增殖能力明显低于对照组，细胞凋亡明显高于对照组(P<0.05)。与对照组相比，柴胡皂苷D组HepG2细胞Ki67表达明显减弱，cleaved caspase-3的表达明显增强(P<0.05)。而且，柴胡皂苷D组裸鼠肿瘤体积明显小于对照组(P<0.05)。柴胡皂苷D处理可提高小鼠存活率。另外，柴胡皂苷D组裸鼠肿瘤组织细胞凋亡远远高于对照组(P<0.001)。与对照组相比，柴胡皂苷D组裸鼠肿瘤组织Ki67表达显著降低，cleaved caspase-3表达显著增强(P<0.01)。结论：柴胡皂苷D可抑制人肝癌HepG2细胞系增殖及裸鼠肝癌形成。

Inhibition of saikosaponin D on cell proliferation of HepG2 cells and tumor growth of liver cancer

Objective:To explore the effect of saikosaponin D(SSd)on cell proliferation of HepG2 cells and tumor growth of liver cancer.Methods: Proliferation of HepG2 cells was measured by CCK-8.Flow cytometrywas performed to test the apoptosis of HepG2 cells.The expression of Ki67 and cleaved caspase-3 in HepG2 cells was detected by Western blot.Terminal deoxynucleotidyltransferase.mediated dUTP nick labeling(TUNEL)was used to test apoptosis of tumor tissues.The expression of Ki67 and cleaved caspase-3 in tumor tissues was measured by immunohistochemical staining.Results: After treating with saikosaponin D,the proliferation of HepG2 cells was decreased with increased apoptosis(P<0.05).Compared with control group,the expression of Ki67 in HepG2 cells was reduced with enhancive expression of cleaved caspase-3(P<0.05).What′s more,the tumor volume in saikosaponin D group was smaller than control group(P<0.05).The survival rate was increased by saikosaponin D.In addition,the apoptosis of tumor tissues in saikosaponin D group was higher than control group(P<0.001)Compared with control group,the expression of Ki67 of tumor tissues in saikosaponin D group was decreased with elevated expression of cleaved caspase-3(P<0.01).Conclusion: Saikosaponin D inhibits the cell proliferation of HepG2 cells and tumor growth of liver cancer