Differential expression of peroxiredoxin 6, annexin A5 and ubiquitin carboxyl-terminal hydrolase isozyme L1 in testis of rat fetuses after maternal exposure to di-n-butyl phthalate |
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| Institution: | 1. Department of Urology, BenQ Medical Center, Nanjing Medical University, 210019 Nanjing, Jiangsu, China;2. Department of Urology, First Affiliated Hospital of Nanjing Medical University, 210029 Nanjing, Jiangsu, China;1. MOE Key Laboratory of Modern Acoustics, Nanjing University, Nanjing 210093, China;2. Department of Nephrology, Nanjing Children''s Hospital, Nanjing Medical University, Nanjing 210008, China;3. Department of Radiology, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA;4. Department of Automation, Nanjing College of Chemical Technology, Nanjing 210048, China;1. Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain;2. Instituto de Química-Física “Rocasolano” (CSIC), Serrano 119, 28006 Madrid, Spain;1. Department of Radiology, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455;2. Department of Urology, University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455;3. Department of Urology, Hospital das Clínicas and University of Sao Paulo Medical School, Sao Paulo, Brazil;4. Department of Radiology, Hospital das Clínicas and University of Sao Paulo Medical School, Sao Paulo, Brazil;5. Department of Radiology, Universidade Nova de Lisboa, Saint Louis Hospital, Lisbon, Portugal;6. Department of Urology, Moores Comprehensive Cancer Center, University of California San Diego Health System, La Jolla, California;7. Division of Urology, Southern Illinois University School of Medicine, Springfield, Illinois;8. Department of Radiology, North Shore Long Island Jewish Health System, Long Island Jewish Medical Center, and Hofstra North Shore Long Island Jewish School of Medicine, New Hyde Park, New York;9. Department of Radiology, MedStar Georgetown University Hospital, Washington, D.C.;10. Department of Radiology, The University of Texas M.D. Anderson Cancer Center, Houston, Texas |
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| Abstract: | ObjectivesTo isolate and identify differentially expressed proteins in testis of rat fetuses after maternal exposure to di-n-butyl phthalate (DBP).MethodsPregnant rats were daily treated by gavage with 1 ml/kg corn oil or 750 mg/kg DBP from GD14 to GD18. We used the technique of proteomic analysis to compare the testis protein patterns obtained by two-dimensional gel electrophoresis from fetal rats of gestation day 19.ResultsWe found significant differences in protein spot intensities compared to control. Subsequently several of these variant protein spots were identified by mass spectrometry. Peroxiredoxin 6 (Prdx6), annexin A5 (AnxA5) and ubiquitin carboxyl-terminal hydrolase isozyme L1 (UchL1) were three of them, the differential expression of which were confirmed by western blotting. Further, immunohistochemical analyses of fetal rat testes sections were made to determine the cellular distribution of these proteins, consequently strong Prdx6 and AnxA5 stainings were found primarily in Leydig cells, while a weak UchL1 staining was found primarily in spermatogonium.ConclusionsThe present study had found several differentially regulated proteins and demonstrated the differential expression of Prdx6, AnxA5 and UchL1 in fetal rat testis after maternal exposure to DBP, when compared with controls. Combining the cellular location of these proteins and their function in other tissues, the results of this study indicated that oxidative injury and abnormal apoptotic regulation might participate the formation of testicular dysgenesis in fetuses of dams exposed to DBP. |
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| Keywords: | Rat Testis Proteomic analysis |
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