A rapid and sensitive method for the determination of dibutyl phthalate in wine by flow-injection chemiluminescence analysis |
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| Institution: | 1. Center for Nano Science and Technology, College of Physics and Electronics Information, Anhui Normal University, Wuhu 241000, China;2. National Laboratory of Solid State Microstructures, Nanjing University, Nanjing 210093, China;3. School of Electronic Science and Engineering, and Key Laboratory of Photonic and Electronic Materials, Nanjing University, Nanjing 210093, China;4. School of Physical Science and Technology, Soochow University, Suzhou 215006, China;1. CNRS, Laboratoire de Chimie de Coordination (LCC) du CNRS, 205 route de Narbonne, BP44099, Toulouse Cedex 4 31077, France;2. Centro de Investigaciones en Óptica (CIO), A.P. 1-948, 37000 León, Gto, Mexico;3. Facultad de Quimica, Departamento de Quimica Orgánica, Universidad Nacional Autónoma de México, 04510 México D.F., Mexico;1. The Key Laboratory of Applied Chemistry of Chongqing Municipality, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China;2. Department of Science and Environmental Studies, The Education University of Hong Kong, 10 Lo Ping Road, Tai Po, Hong Kong SAR, China;1. Department of Pharmaceutical Engineering, Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, 510006, People''s Republic of China;2. Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov State University, Moscow, 119992, Russia |
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| Abstract: | A sensitive method for the determination of picogram level dibutyl phthalate (DBP) in wine by flow-injection chemiluminescence (FI–CL) analysis is presented for the first time, which was based on the quenching effect of DBP on the luminol–myoglobin (Mb) CL system. The decrement of CL intensity was linearly proportional to the logarithm of DBP concentration in the range of 0.1–100 pg mL?1 with the detection limit of 0.03 pg mL?1 (3σ). At a flow rate of 2.0 mL min?1, a complete determination of DBP including sampling and washing could be accomplished in 0.5 min, giving the maximum sample throughput of 120 h?1. The proposed method was successfully applied to the determination of DBP in wine, human serum and urine samples with the relative standard deviations (RSDs) of less than 3.0% (n = 5). The molecule docking results showed that DBP interacted with the amino acid residues near the heme moiety of Mb. The possible CL mechanism of luminol–Mb–DBP reaction should be that the binding of Mb with DBP forming a 1:1 complex (binding constant K = 1.55 × 104 L mol?1) led to the conformational change of Mb and resulted in the quenching of CL intensity. |
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| Keywords: | Food safety Dibutyl phthalate Wine Luminol Myoglobin Chemiluminescence Flow injection Plasticizer Food analysis Organic contaminants in food |
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