鄂温克民族X染色体遗传结构及遗传关系

目的:研究内蒙古鄂温克族人群9个X-STR位点的多态性分布及群体遗传学、法医学应用价值.方法: 选择9个X-STR(DXS6804,DXS7133,DXS101,DXS6789,DXS6799,DXS7423,HPRTB,DXS8378,DXS7132),分析其多态性分布及与其他群体间的遗传距离和聚类关系.结果:研究群体中共检出51种等位基因,频率分布在0.0109～0.6863;基因型频率分布在0.0217～0.3778之间;杂合度、个体识别率、多态性信息量等群体遗传学指标分析显示,9个STR位点具有中度或高度多态性;聚类分析和系统进化关系分析发现,鄂温克族相对于内蒙古地区其他3个民族和西安汉族具有较独立的遗传结构.结论:所选择9个X-STR位点具有较高的个体识别率和多态性信息量,可用于群体遗传学和法医学研究.

Genetic structure of X-STR loci of Ewenki nationality andits affinity with other nationalities

OBJECTIVE: To determine the genetic diversity of X-TR loci, and to evaluate the genetic structure X chromosome's of Ewenki nationality and its affinity with other nationalities. METHODS: We chose 9 X-TR (DXS6804, DXS7133, DXS101, DXS6789, DXS6799, DXS7423, HPRTB, DXS8378, DXS7132) as genetic markers from 99 irrelative individules to determine the genetic diversity of Ewenki in Inner Mongolian. Cluster analysis and phylogenic trees was applied to show the genetic distance among the nationalities.RESULTS: We got 51 alleles in the studied population, with the frequency diverse between 0.0109 and 0.6863.Genotype frequency was from 0.0217 to 0.3778. Heterozygosity(H),the power of discrimination(PD) and the polymorphism information conten (PIC) were consistent with the forensic application. Cluster analysis and phylogenic trees revealed that Ewenki nationality had estrangement genetic affinity with the other 3 major nationalities in inner mongolia and Han nationality in Xi'an. CONCLUSION: The genetic information demonstrates that the 9 chosen gene makers were highly informative loci and are suitable for population genetics research and forensic application.