胡芦巴多糖及低聚糖调血脂活性研究

目的 考察胡芦巴多糖及其酶解产物低聚糖的调血脂活性,并初步探讨其作用机制.方法 体外测定胡芦巴多糖、低聚糖与胆固醇胶束结合的活性;高脂饮食制备高血脂大鼠模型,造模动物分别喂饲拌有胡芦巴多糖或低聚糖(质量分数2.5%)的高脂饲料,给药8周后收集各组大鼠粪便1周,给药9周后取各组大鼠血清和肝脏,检测血清中血脂各项指标及肝功能指标,检测血清中抗氧化活性相关指标,HE染色观察肝组织病理变化,检测粪便中胆固醇及胆汁酸(TBA)的排泄量.结果 胡芦巴多糖及低聚糖体外均表现出一定的胆固醇胶束结合活性;胡芦巴多糖及低聚糖均可降低高血脂大鼠血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)水平,同时升高血清中高密度脂蛋白胆固醇(HDL-C)水平;胡芦巴多糖及低聚糖对高血脂大鼠肝脏脂肪变性有改善作用;同时能提高高血脂大鼠血清超氧化物歧化酶(SOD)活性,促进TBA排泄.结论 胡芦巴多糖及低聚糖均具有明确的调血脂活性,其机制与抗氧化和调节胆固醇-TBA动态平衡有关,调血脂活性酶解产物低聚糖作用较优.

Effects of polysaccharide and oligosaccharide from Trigonellae Semen on dyslipidemia

Objective To investigate the inhibitory effect of polysaccharide (PS) extract from Trigonellae Semen (TS, the seeds of Trigonella foenum-graecum) and its enzymatically digested low molecular weight oligosaccharide (OS) on dyslipidemia, and to preliminarily elucidate the mechanism. Methods The combinative activity of PS and OS on cholesterol micelles was determined in vitro. SD rats were made by high fat diet and used as the hyperlipidemia models, which were given the high fat diet mixed with the complex of PS or OS with the concentration of 2.5% for 8 weeks and then collected the feces of rats in each group for 1 week. The rats were sacrificed after treated for 9 weeks to get the serum and liver. The indexes of blood lipid and antioxidant activity in serum and the indexes of liver function were determined. Histopathology assay of liver tissues was observed by HE staining. The excretion of cholesterol and bile acid in feces were also determined. Results Each drug-dealing group exhibited lowered TC, TG, and LDL-C levels, and elevated HDL-C level in serum. Besides, restored liver tissue could be observed. Moreover, PS and OS exhibited the excellent activity of combining to cholesterol micelles, suppressing superoxide dismutase (SOD) level, and accelerating the excretion of bile acid. Conclusion Both PS and OS of TS, especially the later, show the considerable effects on dyslipidemia.