雪旺细胞源营养神经活性物质对大鼠视网膜节细胞损伤的作用

目的 观察雪旺细胞(Schwann cells,SC)源营养神经活性物质 (SC derived neurotrophic activity,SCNA)对Sprague-Dawly(SD)大鼠视神经损伤后视网膜节细胞(retinal ganglion cells,RGC)存活的影响。 方法 体外培养日龄3～5 d SD乳鼠SC，收集无血清条件培养液，经超滤浓缩后制成冻干粉。SD大鼠分为正常对照组，视神经夹伤对照组，视神经夹伤溶剂对照组，视神经夹伤SCNA 治疗组，每组20只眼。荧光金逆行标记RGC后7 d，除正常对照组外均行球后视神经夹伤，SCNA治疗组将100 ng SCNA注入大鼠玻璃体腔内。分别于视神经夹伤后第5、7、14、21、28 d 将动物灌注固定，做全视网膜铺片，行RGC计数。 结果 视神经夹伤后第7 d RGC开始减少，14 d时降至正常对照的70.2%，28 d时降至40.5%。SCNA治疗组7 d时RGC数开始减少，但14、21、28 d RGC数均明显多于视神经夹伤对照组及视神经夹伤溶剂对照组(P＜0.01)。 结论 在视神经夹伤后眼内注射SCNA能减少RGC的死亡对RGC损伤有保护作用。（中华眼底病杂志，2000，16：1-70）

Effects of schwann cell-derived neurotrophic activity on injured retinal ganglion cells of rats

Objective To investigate the survivability of ret inal ganglion cells (RGC) after optic nerve crush with intraocular injection of schwann cells(SC) derived neurotrophic (SCNA) in vivo． Methods Schwann cells of 3～5 day newborn mice were cultured,conditioned media without serum was collected,ultraspeed centrifugalized,and frozen-dry.SD rats were divided into normal contrl,crush control,medium treatment and SCNA treatment groups,and 20 eyes in every group.RGC of adult rats were labelled with flu orogold.Seven days later,the optic nerve was intraorbitally crushed and SCNA was injected into the vitreous on the 5th,7th,21th and 28th day after crush,the number of RGC were counted respectively. Results The densities of RGC began to decrease on the 7th day after injury,the number of RGC was 70.2% and 40.5% of normal controls on the 14th and 28th day,respectively .In the group with SCNA injection,RGC densities decreased on the 7th day,but RGC densities were much higher then that of controls on the 14th,21th,and 28th day after injury (P<0.01). Conclusions SCNA administered intraocularly at the time of crush of optic nerve can protect RGC from injury and death of the cells.(Chin J Ocul Fundus Dis，2000，16：1-70)