脉冲等离子体涂层神经导管修复周围神经缺损实验研究

目的:探讨经脉冲等离子体涂层并固定睫状神经营养因子(CNTF)的聚羟基乙酸和聚乳酸共聚物(PGLA)神经导管修复周围神经缺损的效果.方法:应用脉冲等离子方法对PGLA膜片或神经导管表面进行处理,然后固定CNTF.该膜片表面种植人胚胎视神经细胞,经处理的神经导管修复SD大鼠坐骨神经1.5 cm缺损.观察人胚胎视神经细胞在经处理的膜片上生长情况,并用电生理和轴突计数法评价经处理的神经导管内神经再生质量.设立未固定CNTF的PGLA膜片组和未经过脉冲等离子体涂层的神经导管组作为对照组比较.结果:在经处理的PGLA膜片上,人胚胎视神经细胞生长较对照组密集,且发现有轴突延伸相接现象.经处理的PGLA修复大鼠坐骨神经缺损,在1个月和3个月时,均发现神经导管内神经传导速度和再生神经轴突均明显大于对照组(P＜0.05).结论:经脉冲等离子体涂层并固定睫状神经营养因子(CNTF)的PGLA神经导管可能通过CNTF的接触诱导和持续缓释作用,促进周围神经再生.

Experimental study on repair of peripheral nerve defects using pulsed plasma coated nerve conduit

Objective: To investigate the result of pulsed plasma coated and ciliary neurotrophicfactor (CNTF) fixed PGLA nerve conduits to repair peripheral nerve defects. Methods: PGLA mem-brane or PGLA nerve conduits were treated with pulsed plasma and then fixed with CNTF. Humanembryonic optical nerve cells were cultured on the PGLA membrane. The treated PGLA nerveconduits were used to bridge a 1.5cm nerve defect in a SD rat sciatic nerve model. The growth ofthe embryonic optical cells on the membrane was observed. The nerve regeneration within thePGLA nerve conduits was determined with electrical conduction and axon counting. The untreatedPGLA membrane and conduits were acted as control groups. Results: On the treated PGLAmembrane, human embryonic optical nerve cells grew more densely compared to the control group,furthermore, the extension of axons was found on the treated PGLA membrane. One month andthree months after repair of the rat sciatic nerve gaps, it was found that the nerve conduction veloc-ity and the number of regenerated axons were significantly greater in the treated PGLA nerve con-duits than the untreated ones (P<0.05). Conclusion: It indicated that pulsed plasma coated and theCNTF fixed PGLA nerve conduits can improve peripheral nerve regeneration, possible due to thecontact guidance and persistent release of CNTF.