Glucosamine-resistant mutations in yeast affecting the glucose repression sensitivity of electron transport enzymes |
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Authors: | Saroj D. Mishra Corinne A. Michels |
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Affiliation: | (1) Department of Biology, Queens College of C.U.N.Y., 11367 Flushing, NY, USA;(2) Present address: Biochemical Engineering Research Centre, Indian Institute of Technology, Delhi, Hauz Khos, 110016 New Delhi, India |
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Abstract: | Summary We have isolated two mutant strains, GSAr-4 and GSAr-5, which are able to grow on lactate in the presence of D-glucosamine. The glucosamine-resistant phenotype results from the cooperative effects of mutations in three loci, GAR1, GAR2 and GAR3. Both glucosamine resistant mutant strains were doubly mutant at gar1 gar2 (GSAr-4) or gar1 gar3 (GSAr-5). The mutants were also shown to exhibit glucose repression insensitive synthesis of NADH-cytochrome c reductase and cytochrome c oxidase. Glucose-repressible synthesis of the following enzymes was seen: succinic dehydrogenase, succinic: cytochrome c reductase, maltase (PNPGase), galactokinase, -galactokinase. The glucose-repression insensitivity segregates in association with the glucosamine resistance. |
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Keywords: | Glucosamine-resistant mutants Glucose repression |
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