电针对豚鼠肾上腺环核苷酸免疫反应性的影响

应用PAP法对电针组(足三里穴)和对照组共38只豚鼠肾上腺环—磷酸腺苷免疫反应性(cAMP-IR)和环—磷酸乌苷免疫反应性(cGMP—IR)进行了观察。免疫阳性反应呈棕黄色颗粒,主要分布在细胞质和细胞膜内。电针组与对照组比较观察结果可见:电针组肾上腺cAMP—IR和cGMP—IR有显著性增强(P?0.05),而髓质cAMP—IR和cGMP-IR有增强,但不明显(P?0.05)。两组皮质cAMP—IR和cGMP—IR均比髓质强(P?0.05)。以上结果可表明电针“足三里”穴能引起肾上腺cAMP—IR和cGMP—IR显著性增强,髓质有增强。本文还对肾上腺cAMP-IR和cGMP-IR与髓质脑啡肽的相关性进行了分析。

The effect of electroacupuncture on the immunoreactivity cyclic nucleotides in the adrenal gland of guineca pigs

In order to study the electroacupuncture effect on the cyclic nucleotides in theadrenal gland, and its relation with the founction in the adrenal gland, cAMP-IR(Cyclic Adenosine-3', 5' Monophosphate Immunoreactivity) and cGMP-IR (CyclicGuansine-3', 5' Monophosphate Immunoreactivity) were observed with PAP immu-nochemical technique in the adrenal gland of 38 guinea pigs, which were dividedinto the electroac upuncture group and the control group. The positive reactivity pre-sented brown granules, which were scattered in the cytoplasm and the cell membrane.The comparison of the electroacupuncture group with the control group showed thatcAMP-IR and cGMP-IR were significantly increased in the adrenal cortex ofthe guinea pigs of the electroacupuncture group(P<0.05), while there was no signi-ficant increase in the adrenal medulla(P 0.05). The cAMP-IR and cGMP-IR inthe adrenal cortex in both groups were stronger than those in the adrenal medulla (P<0.05). The results mentioned above indicate that the electroacupuncture of "zusan-li" points may lead to the significant increase of cAMP-IR and cGMP-IR in theadrenal cortex. The relation between cGMP-IR and cGMP-IR and the relationbetween cyclic nucleotides and enkephalin in adrenal gland were also analyzed inthis paper.