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| 导流杂交基因芯片技术在肠道致病菌检测中的应用研究 | |
| 引用本文： | 邢建明,张甦,周丰宁,张红河,沈翠芬,吴晓芳,毕丹,程红玲,姚丽惠.导流杂交基因芯片技术在肠道致病菌检测中的应用研究[J].中华医院感染学杂志,2008,18(10). |
| 作者姓名： | 邢建明 张甦 周丰宁 张红河 沈翠芬 吴晓芳 毕丹 程红玲 姚丽惠 |
| 作者单位： | 1. 湖州市妇幼保健院检验科,浙江,湖州,313000 2. 杭州市第一人民医院基因诊断实验室,浙江,杭州,310006 3. 湖州市中心医院检验科,浙江,湖州,313000 4. 湖州市疾病预防控制中心,浙江,湖州,313000 |
| 摘 要： | 目的建立导流杂交芯片技术检测粪便标本中8种肠道致病菌。方法以细菌16S rDNA和23S rDNA序列设计通用引物和寡核苷酸探针,将活性氨基标记的探针依次固定在BiodyneC膜上,制成检测芯片,用标记生物素的引物进行PCR扩增,将扩增产物与检测芯片在核酸分子快速杂交仪上进行杂交,以POD和TMB进行显色,判断结果;应用该方法检测8种肠道致病菌标准菌株和120份腹泻患者粪便标本,通过细菌培养结果比较该方法的有效性。结果120份临床粪便标本中,采用导流杂交芯片共检出68份（56.67%）携带肠道致病菌;细菌培养鉴定出63份（52.50%）携带肠道致病菌,其中58份导流杂交芯片与细菌培养结果一致,准确率为85.29%。结论导流杂交芯片技术能够快速准确检测8种常见肠道致病菌,而且适用于流行病学调查研究。 |
| 关 键 词： | 基因芯片 肠道致病菌 检测 |
Flow-through Hybridization and Gene Chip in Intestinal Pathogens Detection:An Applied Research |
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| XING Jian-ming,ZHANG Su,ZHOU Feng-ning,ZHANG Hong-he,SHEN Cuif-en,WU Xiao-fang,BI Dan,CHENG Hong-ling,YAO Li-hui.Flow-through Hybridization and Gene Chip in Intestinal Pathogens Detection:An Applied Research[J].Chinese Journal of Nosocomiology,2008,18(10). | |
| Authors: | XING Jian-ming ZHANG Su ZHOU Feng-ning ZHANG Hong-he SHEN Cuif-en WU Xiao-fang BI Dan CHENG Hong-ling YAO Li-hui |
| Institution: | XING Jian-ming*,ZHANG Su,ZHOU Feng-ning,ZHANG Hong-he,SHEN Cui-fen,WU Xiao-fang,BI Dan,CHENG Hong-ling,YAO Li-hui(*Microbiology Laboratory,Huzhou Maternity , Child Care Hospital,Huzhou,Zhejiang 313000,China) |
| Abstract: | OBJECTIVE To establish a flow-through hybridization and gene chip method for detecting eight intestinal pathogens.METHODS Biotin-labeled universal primers for amplification of bacterial 16S rDNA and 23S rDNA were used to amplify extracted genome DNA.The eight intestinal pathogens were identified by hybridization of the PCR products with immobilized specific probes on BiodyneC membrane in rapid hybridization machine.Color development was achieved by adding POD and TMB.A judgement was made according to color ... |
| Keywords: | Gene chip Intestinal pathogen Detection |
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