超低温处理后的兔颈总动脉血管支架内皮化的实验研究

为了构建理想的血管移植物,解决移植后由于超急性排斥反应的发生而引起的血栓问题,本文采用超低温慢冻速融法处理兔颈总动脉的方法去除血管内腔的内皮细胞,并保留内皮细胞下的其他组织结构,然后将原代培养的人脐静脉内皮细胞高密度种植于去掉内皮细胞的血管内腔面.银染和HE染色鉴定的结果表明血管支架在体外达到了很好的内皮化程度.本研究为组织工程血管的异种移植提供了可靠方法学的依据,对组织工程血管的成功构建具有十分重要的意义.

Endothelialization of Treated Rabbit Common Carotid Artery by Super Low Temperature

The study on constructing appropriate blood vessel graft and solving the thrombus caused by originated hyperacute rejection have been increased up to date.The method of hyper-cryopreservation introduced in the present does intravascular velum of rabbit common carotid artery treated without the native endothelial cell layer retained,but with the native smooth muscle cells layer. Human umbilical vein endothelial cells (a gift from the friendly hospital) cultured with high density are implanted on the above treated intravascular space. The results of silver staining and HE staining indicated that intravascular velum form integrate endothelial cell layer in vitro. Meanwhile the research provides a potential way for heterogenic transplantation of blood vessel tissue engineering, and is also important to succeed construct tissue-engineered blood vessel.