1725例血清抗核抗体及抗核抗体谱检测结果分析

目的探讨抗核抗体（ANA）和ANA谱在自身免疫性疾病（AID）中的临床价值及相符程度。方法分析1725例标本血清ANA及ANA谱检测结果。ANA和抗双链DNA抗体采用间接免疫荧光法（IIF）,ANA谱采用欧蒙印迹法。结果 1725例标本血清ANA阳性共768例,阳性率为44.52%。荧光模式主要为核颗粒型、核均质型、核仁型等;1725例标本血清ANA谱检测有782例阳性,阳性率为45.33%,大多为抗体二联及以上共同出现阳性,阳性抗体出现较多的是抗SS-A、抗Ro-52、抗SS-B、抗Histone等。结论 ANA和ANA谱联合检测在AID诊断中具有互补性,可提高检出率,对AID的诊断分型、治疗、预后判断和病情随访等均有重要的临床价值。

Analysis of the result of antinuclear antibody and its profile for 1725 patients

Objective To explore the clinic value and the extent consistent of antinuclear antibodies(ANA) and antinuclear antibody profile in the autoimmune diseases(AID).Methods Retrospective analysis of the result of ANA and ANA profile in sera of 1725 patients.ANA and anti-double-stranded DNA antibody were detected by indirect immunofluorescence(IIF),ANA profile were detected by Euronline(immunoblot assay).Results 768 ANA positive cases were found out of the 1725 patients,the positive rate being 44.52%.The main fluorescent type of ANA were nuclear particles pattern,nuclear homogenous pattern,nucleolar pattern.Of all the 1725 samples,782 cases is positive for ANA profile.The positive rate is 45.33%.Most of the antibodies appear together two or more positive joint,the most frequently occurred antibody was anti-SS-A,anti-Ro-52,anti-SS-B and anti-Histone.Conclusion ANA and ANA profile can supplement each other in diagnosing AID and increase the detection rate,which is of great clinic value to diagnostic classification,treatment,post-prediction judge and disease follow-up of AID.