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| NADPH氧化酶源性ROS在乳鼠心肌细胞肥大中的作用 | |
| 引用本文： | 苏兴利,张鸿,霍健,王爽,郭玉芳,王湘,王多宁,杨颖. NADPH氧化酶源性ROS在乳鼠心肌细胞肥大中的作用[J]. 西部医学, 2012, 24(3): 450-452 |
| 作者姓名： | 苏兴利 张鸿 霍健 王爽 郭玉芳 王湘 王多宁 杨颖 |
| 作者单位： | 1. 西安医学院基础医学部病理生理教研室,陕西西安,710021 2. 陕西省人民医院传染科,陕西西安,710068 3. 西安医学院科研中心,陕西西安,710021 |
| 摘 要： | 目的探讨NADPH氧化酶来源的活性氧(ROS)在TNF-α诱导乳鼠心肌细胞肥大中的作用。方法采用体外乳鼠心肌细胞培养,给予TNF-α、APO单独或联合作用细胞24h,以BCA法测定心肌细胞蛋白合成,共聚焦显微镜测量心肌细胞ROS含量,RT-PCR法测定心肌细胞心钠素ANP mRNA的表达。结果 TNF-α可明显增加心肌细胞蛋白合成,心肌细胞体积增大,ROS生成增多,ANP mRNA表达水平显著增加,以上作用可被APO明显抑制。结论TNF-α通过增加心肌细胞NADPH氧化酶来源的ROS生成,促进心肌细胞肥大。 |
| 关 键 词： | 心肌细胞 ROS TNF-α 心肌肥大 NADPH氧化酶 |
Role of NADPH oxidases derived ROS in neonatal cardiomyocyte hypertrophy |
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| Affiliation: | SU Xing-li,ZHANG Hong,HUO Jian,et al(Department of Pathophysiology,Xi’an Medical University,Xi’an 710021,China) |
| Abstract: | Objective To explore the role for ROS-generating NADPH oxidases in neonatal cardiomyocytes hypertrophy.Methods Primary cultured fetal rat cardiomyocytes were treated with TNF-α,apocynin,or TNF-α+APO.The protein content of myocyte was detected by BCA method.The DCFH-DA sensitive cellular ROS was measured by fluorometric assay and confocal microscopy.The ANP mRNA expression detected by RT-PCR.Results TNF-α treatment of the cardiomyocytes resulted in significantly increased ROS pruduction,protein synthesis,ANP mRNA expression in crdiomyocytes,and induced cardiomyocytes hypertrophy.The effects were inhibited by application of APO.Conclusion NADPH oxidase-dependent ROS mediates cardiomyocytes hypertrophy induced by TNF-α. |
| Keywords: | Cardiomyocyte ROS TNF-α Myocardial hypertrophy NADPH oxidase |
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