| 抑癌基因p16^INK4真核表达转移载体的构建 |
| |
| 引用本文： | 朱长军 张利宁. 抑癌基因p16^INK4真核表达转移载体的构建[J]. 泰山医学院学报, 2000, 21(1): 4-6 |
| |
| 作者姓名： | 朱长军 张利宁 |
| |
| 作者单位： | 泰山医学院免疫学教研室!山东泰安271000(朱长军)，山东医科大学微生物免疫学教研室!山东济南250012(张利宁，马春红，曹英林)，山东省泰山疗养院!山东泰安271000(赛宁) |
| |
| 基金项目： | 卫生部科研基金资助项目!(4 13653) |
| |
| 摘 要： | 目的 构建可应用于昆虫杆状病毒表达系统的含抑癌基因p16 INK4cDNA的重组转移载体。方法 采用定向克隆方法将抑癌基因p16 INK4cDNA全长插入转移载体质粒pSXIVVI X3,并经斑点杂交 ,PCR及酶切鉴定重组质粒。结果 经三种鉴定方法证实p16 INK4cDNA片段成功地插入转移载体pSXIVVI X3。结论 重组质粒pSXIVVI X3 p16构建成功 ,为真核表达抑癌基因p16 INK4奠定了基础。
|
| 关 键 词： | 抑癌基因 p16^INK4 肿瘤 转移载体 真核表达 |
Construction of eukaryotic transfer vector of tumor suppressor gene p16~( INK4) |
| |
| ZHU Chang jun ,ZHANG Li ning ,MA Chun hong ,CAO Ying lin ,SAI Ning. Construction of eukaryotic transfer vector of tumor suppressor gene p16~( INK4)[J]. Journal of Taishan Medical College, 2000, 21(1): 4-6 |
| |
| Authors: | ZHU Chang jun ZHANG Li ning MA Chun hong CAO Ying lin SAI Ning |
| |
| Affiliation: | ZHU Chang jun 1,ZHANG Li ning 2,MA Chun hong 2,CAO Ying lin 2,SAI Ning 3 |
| |
| Abstract: | Objective: To obtain the eukaryotic expression transfer vector containing the tumor suppressor gene p16 INK4 cDNA. Methods:By directional cloning method, p16 INK4 cDNA fragment was cloned into the transfer vector pSXIVVI X 3 and the recombinant plasmid DNA was identified with dot blot hybridization, PCR, and enzyme digestion. Results: p16 INK4 cDNA fragment had been cloned into the transfer vector pSXIVVI X 3 successfully. Conclusion: The recombinant plasmid pSXIVVI X 3 p16 is constructed successfully, which lays a good basis for expressing p16 INK4 gene with insect bacularvirus expression system. |
| |
| Keywords: | tumor suppressor gene p16~(INK4) insect bacularvirus expression system transfer vector |
| 本文献已被 CNKI 维普 等数据库收录！ |
