反复热性惊厥对大鼠行为运动及空间学习记忆的影响

背景热性惊厥脑损伤已经引起关注,但热性惊厥对行为运动及学习记忆影响的研究也是十分重要的.目的探讨热性惊厥对大鼠行为运动及空间学习记忆能力的影响.设计随机对照的实验研究.地点和材料实验在西安交通大学医学院实验动物中心完成.实验选用21日龄雄性SD大鼠60只,按随机数字法均分为热性惊厥组、发热对照组及正常对照组.干预45℃热水浴建立热性惊厥动物模型,定时浸浴2 min设立发热对照组.通过斜板试验、悬吊试验、旷场活动试验及Morris水迷宫检测大鼠行为运动及空间学习记忆能力变化.主要观察指标各组大鼠转体时间、悬吊时间、旷场活动性、逃逸潜伏期及搜寻策略比较.结果多次热性惊厥发作可使大鼠在斜板试验热性惊厥组为(9.15±2.63)s,发热对照组为(5.32±2.11)s,正常对照组为(5.29±2.04)s,热性惊厥组和其他两组比较,差异有显著性意义(F=6.17,P＜0.01)],悬吊试验热性惊厥组为(33.4±18.1)s,发热对照组为(50.1±20.3)s,正常对照组为(59.0±20.7)s,热性惊厥组与其他两组比较,差异在显著性意义(F=8.23,P＜0.01)],旷场活动试验热性惊厥组为(5.07±2.04)分,发热对照组为(10.37±2.98)分,正常对照组为(13.22±2.26)分,热性惊厥组与其他两组比较,差异在显著性意义(F=7.42,P＜0.01)]中行为运动能力下降.在Morris水迷宫试验中,热性惊厥组逃逸潜伏期延长,平台所在区域内的游泳时间百分比降低以及穿过平台面积的次数减少.结论热性惊厥发作可损害大鼠的行为运动和空间学习记忆能力.

Effects of repeated febrile convulsion on behavior motion,spatial learning and memory in rats

BACKGROUND:Although researchers home and abroad have paid attention to studies on brain damage possibly caused by febrile convulsion, studies of effects on behavior motion, learning and memory are relatively seldom.OBJECTIVE:To study the effects of repeated febrile convulsion on behavior motion, spatial learning and memory in rats.DESIGN:Randomized controlled experiment.SETTING and PATICIPANTS:All experiments were performed in the Laboratory Animal Center of Xi' an Jiaotong University.Sixty 21-day-old male Sprague-Dawley rats were randomized into febrile convulsion group (FC, n = 20), hypothermic control group (HC, n = 20), normal control group (NC, n = 20).INTERVENTIONS:FC model was induced by hyperthermal bath with 45 ℃water.HC model which rats were bathed with the same water in fixed 2 minutes was also developed in this study.The abilities of motor and behavior were tested by inclined plane test (IPT), overhanging test(OHT) and open field test (OFT) .Morris water maze(MWM) was applied to show the variations in abilities of spatial learning and memory in each group.MAIN OUTCOME MEASURES:The indexes included turning time,overhanging time, activity, escape latency and search strategy.RESULTS:Repeated febrile convulsion could lead to decreases of abilities in IPT(9.15±2.63) s for FC, (5.32±2.11) s for HC and(5.29±2.04) sfor NC.FC vsHCorNC, F=6.17, P ＜0.01], OHT(33.4±18.1) sforFC,(50.1±20.3) sforHC and(59.0±20.7) s forNC.FC vs HC orNC, F=8.23, P ＜ 0.01 ] and OFT (5.07 ± 2.04) scores for FC, (10.37 ± 2.98)scores for HC and(13.22 ± 2.26) scores for NC, FC vs HC or NC, F = 7.42,P ＜ 0.01 ] .In MWM test, the escape latency prolonged, the percentage of swim time in platform quadrant reduced and the times of swimming across platform area decreased in FC group.CONCLUSION:Febrile convulsion can impair behavior motion, spatial learning and memory in rats.