吗啡后处理对大鼠缺血再灌注心肌线粒体膜通透转运孔的影响

目的 观察吗啡后处理对雄性大鼠缺血再灌注心肌细胞线粒体膜通透转运孔（MPTP）的影响。方法 S‐D雄性大鼠48只，体质量170～260 g ，随机分为4组（ n ＝12）：①假手术组（C组），②缺血再灌注组（I／R组），③吗啡后处理组（MP组），④吗啡＋苍术苷组（MAP组）。采用结扎左冠状动脉前降支30 min再灌注90 min的方法建立心肌缺血再灌注损伤模型。再灌注结束后，HE染色观察心肌细胞结构，检测心肌梗死区／缺血区（IS／AAR）梗死百分比、心肌组织丙二醛（MDA）含量。结果 与I／R组比较，MP组血流动力学显著改善，心肌梗死百分比 IS／AAR％和 MDA 含量显著降低，而 MAP组与 MP组比较，心肌梗死面积（IS／AAR）和MDA含量显著增高。结论 吗啡后处理可显著减轻心肌缺血再灌注损伤；线粒体膜通透性转运孔M PT P可能是吗啡后处理心肌保护的作用通路。

Effect of Morphine Postconditioning on Myocardial Mitochondrial Permeability Transition Pore of Rats with Myocardial Ischemia-reperfusion Injury

Objective] To observe the effect of morphine postconditioning on myocardial mitochondrial permeability transition pore（MPTP） in male rats with myocardial ischemia‐reperfusion injury .Methods]A total of 48 S‐D male rats with weight 170~260g were randomly divided into sham operation group（group C） , ischemia reperfusion group（I/R group） ,morphine postconditioning group（MP group） and morphine plus at‐ractyloside group（MAP group） with 12 rats in each group .Myocardial ischemia/reperfusion injury model was established by ligation of remus descendens anterior arteriae coronariae sinistrae for 30min and reperfusion for 90min .After reperfusion ,myocardial cell structure was observed by HE staining method .The percentage of myocardial infarction area/infarction ischemia area（IS/AAR） ,myocardial malondialdehyde（MDA） in myocar‐dium were measured .Results] Compared with I/R group ,hemodynamics of MP group were markedly im‐proved ,and IS/AAR% and MDA content were markedly increased .Conclusion] Morphine postconditioning can significantly attenuate myocardial ischemia‐reperfusion injury .MPTP may be the action pathway of mor‐phine postconditioning for protecting the myocardium .