重组人粒细胞集落刺激因子动员对CD4+T淋巴细胞迁移和黏附功能的影响

目的探讨重组人粒细胞集落刺激因子(rhG-CSF)动员对CD4+T淋巴细胞表面分子CXCR4和淋巴细胞功能相关抗原1(LFA-1)所介导功能和相关信号机制的影响.方法在rhG-CSF动员前和动员后第5天抽取供者外周血,用三色荧光标记检测动员前后CD4+T淋巴细胞LFA-1和CXCR4的表达率,并应用免疫磁性分选法分离纯化CD4+T淋巴细胞,检测动员前后CD4+T淋巴细胞对基质细胞衍生因子1α(SDF-1α)的迁移能力和对细胞间黏附分子1(ICAM-1)的黏附能力.结果rhG-CSF动员前后CD4+T淋巴细胞的LFA-1(CD11a)和CXCR4表达率差异无统计学意义(P＞0.05),动员前后CD4+T细胞LFA-1表达率均为100%;动员前CD4+T淋巴细胞CXCR4表达率为(84.58±20.31)%,动员后为(81.23±22.46)%.动员前后CD4+T淋巴细胞向SDF-1α的4 h迁移率分别为(28.5±10.3)%和(31.2±8.9)%,差异无统计学意义(P＞0.05);动员前后CD4+T淋巴细胞在CD3单抗作用下对ICAM-1的黏附率分别为(85.59±14.21)%和(61.45±15.07)%,动员前显著高于动员后(P＜0.05).结论rhG-CSF动员不影响CD4+T淋巴细胞LFA-1和CXCR4的表达,但影响CD4+T淋巴细胞通过LFA-1对ICAM-1的黏附能力.

The impact of rhG-CSF mobilization on migration and adhesive function of CD4 + T cells

OBJECTIVE: To explore the impact of mobilization with recombinant human granunocyte clonony stimulated factor (rhG-CSF) on the migration and adhesive function and their related signal mechanism mediated by the CXCR4 and lymphocyte function antigen-1 (LFA-1) molecules on the surfaces of CD4(+) T cells. METHODS: Before and at day 5 on rhG-CSF mobilization, the expression rates of CXCR4 and LFA-1 (CD11a) on CD4(+) T cells in the peripheral blood were detected by tricolor fluorecence labeling, and the migration and adhesive activities of CD4(+) T cells to stroma cell-derived factor 1alpha (SDF-1alpha) and intercellular adhesion molecule-1 (ICAM-1) were also tested. RESULTS: The expression of CXCR4 on CD4(+) T lymphocytes was (84.58 +/- 20.31)% before mobilization and (81.23 +/- 22.46)% at day 5 on mobilization. The expression of LFA-1 on CD4(+) T lymphocytes before and at day 5 on mobilization was 100%. There was no significant difference in the expression CXCR-4 and LFA-1 on CD4(+) T lymphocytes whether mobilization (P > 0.05). SDF-1alpha induced 4 hours' CD4(+) T cells migration didn't change markedly before and after mobilization \(28.5 +/- 10.3)% vs (31.2 +/- 8.9)%\] (P > 0.05). The adhesive activity of CD4(+) T cells to ICAM-1 was decreased from (85.59 +/- 14.21)% to (61.45 +/- 15.07)% after mobilization (P < 0.05). CONCLUSIONS: The expression of CXCR4 and LFA-1 on CD4(+) T lymphocytes didn't change markedly during rhG-CSF mobilization, but the adhesive activity of CD4(+) T cells to ICAM-1 was frustrated after that.