非免疫性过敏反应细胞模型的建立

目的:通过非免疫性刺激物C48/80诱导大鼠嗜碱性粒细胞白血病细胞株RBL-2H3细胞脱颗粒反应正交试验,优化非免疫性过敏反应细胞模型建立条件.方法:在不同的孵育时间下,C48/80与不同浓度的RBL-2H3细胞共孵育,通过测定β-氨基己糖苷酶的释放率确定建立非免疫型过敏反应细胞模型的最优实验条件,并且分析不同检测方法间的差异.结果:不同浓度、不同孵育时间下的RBL-2H3细胞均可受C48/80诱导发生典型的脱颗粒反应,但不同条件下的脱颗粒程度和β-氨基己糖苷酶释放率都有显著差异.结论:当RBL-2H3细胞浓度为2×105 mL、孵育时间60 min时,细胞的感受性较好,其脱颗粒程度与药物浓度呈正相关.

Anaphylactoid reaction based on RBL-2H3 cell modelin vitro

AIM: To observe the effects of C48/80 on RBL-2H3 cell degranulation orthogonal test,optimize the experimental conditions and discuss the possible detection methods of anaphylactoid reaction.METHODS: The β-hexosaminidase assay of RBL-2H3 cells incubated with C48/80 were tested by orthogonal test considering two laboratorial factors time and cell concentration.According the results the optimized cell model in vitro were set up and disscus the superior and the inferior aspects between different detection means.RESULTS:Different concentrations and time could make significance difference on RBL-2H3 cell model with degranulation.CONCLUSION: Anaphylactoid reaction based on RBL-2H3 cell model in vitro could be built up on the cell concentration of 2×105 under 60 minutes incubation time,which may be appropriate conditions in order to get stable cell model because the degranualtion was significantly increased and dose-dependent under this condition.