| 大鼠原位肠肝血管灌流模型的优化及完善 |
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| 引用本文： | 王素军,莫李立,杨本坤,臧林泉,潘雪刁,王桂香,谭毓治,谢海棠. 大鼠原位肠肝血管灌流模型的优化及完善[J]. 中国临床药理学与治疗学, 2012, 17(4): 361-366 |
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| 作者姓名： | 王素军 莫李立 杨本坤 臧林泉 潘雪刁 王桂香 谭毓治 谢海棠 |
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| 作者单位： | 1. 广东药学院药科学院药理系,广州510006,广东
2. 安徽省药物临床评价中心,皖南医学院附属弋矶山医院,芜湖241001,安徽 |
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| 基金项目： | 国家自然科学基金(81073141);2010年科技部新药重大专项(2011zx09102-001-31);广东省自然科学基金(9152402301000007) |
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| 摘 要： | 目的:对大鼠原位肠肝血管灌流模型的灌流液组成成分进行优化,以获取最佳灌流液配方。方法:对灌流条件进行优化,分别对Krebs-Rin-ger(K-R)液加牛血清白蛋白和甘露醇、K-R液加牛血清白蛋白和右旋糖酐T-40二者进行对比,找出最佳的灌流介质。然后对最佳的灌流介质中牛血清白蛋白的含量进行了优化。结果:K-R液加牛血清白蛋白和右旋糖酐T-40组优于K-R液加牛血清白蛋白和甘露醇组;5%牛血清白蛋白的灌流液是一个较为经济理想的灌流液。结论:在灌流介质中5%牛血清白蛋白是一个较为理想和经济的比例。
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| 关 键 词： | 大鼠原位肠肝血管灌流模型 右旋糖酐T-40 甘露醇 牛血清白蛋白 |
Optimization of in situ perfused rat intestine-liver model |
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| WANG Su-jun , MO Li-li , YANG Ben-kun , ZANG Lin-quan , PAN Xue-diao , WANG Gui-xiang , TAN Yu-zhi , XIE Hai-tang. Optimization of in situ perfused rat intestine-liver model[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(4): 361-366 |
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| Authors: | WANG Su-jun MO Li-li YANG Ben-kun ZANG Lin-quan PAN Xue-diao WANG Gui-xiang TAN Yu-zhi XIE Hai-tang |
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| Affiliation: | 1 Department of Pharmacology,Guangdong Pharmacy University,Guangzhou 510006,Guangdong,China;2 Anhui Provincial Center for Drug Clinical Evaluation & Wannan Medical College Affiliated Yijishan Hospital,Wuhu 241001,Anhui,China |
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| Abstract: | AIM: To optimize perfusate composition of in situ perfused rat intestine-liver model and achieve the best perfusate formula.METHODS: The perfusion conditions were optimized,respectively.The Krebs-Ringer NaHCO3 solution with bovine serum albumin and mannitol and the Krebs-Ringer NaHCO3 solution with bovine serum albumin and dextran two T40 were contrasted for finding out the best perfusate medium.Then bovine serum albumin content in the optimal perfusion medium was optimized.RESULTS:K-R solution with bovine serum albumin and dextran T40 group was better than K-R solution with bovine serum albumin and mannitol group;5% bovine serum albumin in perfusate is a more economic and ideal proportions.CONCLUSION: 5% bovine serum albumin in perfusate is a more economic and ideal proportions. |
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| Keywords: | In situ perfused rat intestine-liver model Dextran T40 Mannitol Bovine serum albumin |
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