胺碘酮对SD大鼠甲状腺功能及形态影响的实验研究

目的:探讨胺碘酮对SD大鼠甲状腺组织的激素分泌、信号蛋白表达及形态结构等影响。方法:通过对胺碘酮低剂量组(50mg.kg-1.d-1)及高剂量组(200mg.kg-1.d-1)的SD大鼠14周的饲养,取静脉血测游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、总蛋白(TP)、甘油三酯(TG),取甲状腺组织观察Bcl-2蛋白的表达及Caspase-3蛋白活性高低,并进行组织学检查。结果:用药后SD大鼠外观出现脱毛、行动迟缓、体重下降,血清总蛋白下降;FT3随胺碘酮剂量的增大而下降,FT4在低剂量组略有增高,而在高剂量组则下降;Bcl-2蛋白表达在用药组均增强,而凋亡效应因Caspase-3活性无差异。结论:胺碘酮对SD大鼠甲状腺的影响主要表现为甲状腺激素代谢受抑制,且随剂量的增大而明显,此时甲状腺组织Bcl-2蛋白表达增强,而Caspase-3细胞凋亡效应因子尚未启动。

Experimental study on the effects of amiodarone on thyroid function and morphology in SD rats

AIM:To investigate the effects of amiodarone on thyroid hormone secretion,signaling protein expression and morphology.METHODS: SD rats were divided into low-dose group(50 mg·kg-1·d-1) and high-dose group(200 mg·kg-1·d-1),feeding amiodarone for 14 weeks respectively.Free triiodothyronine(FT3),free thyroxine(FT4),total protein(TP),glycerin three greases(TG) were measured from SD rats’ blood.Analysis of the expression for Bcl-2 protein and the activity of Caspase-3 protein were observed from SD rats’ thyroid gland,and pathology examination were performed.RESULTS:After amiodarone medication,SD rats’ appearance were hair removal,sluggish,weight loss,serum total protein decreased,FT3 decreased with amiodarone’s dose increasing,FT4 in the low-dose group slightly increased,while in the high-dose group decreased.The positive expression of Bcl-2 in low-dose group and high-dose group,while negative expression of Caspase-3 in all of them(P>0.05).CONCLUSION: Effects of amiodarone on SD rats’ thyroid were manifested as thyroid hormone metabolism were inhibited with the dose increasing,and the expression of Bcl-2 protein were enhanced,while the apoptosis by Caspase-3 induced had not been initial.