鸢尾黄素对肝星状细胞外基质表达的影响

目的:研究鸢尾黄素对肝星状细胞的活化以及细胞外基质合成和降解的影响。方法:体外培养大鼠肝星状细胞株HSC-T6,通过荧光定量PCR法检测鸢尾黄素对肝星状细胞的Ⅰ型胶原、α-肌动球蛋白(α-SMA)、基质金属蛋白酶-13(MMP-13)以及基质金属蛋白酶-1抑制剂(TIMP-1)mRNA的表达的影响。结果:鸢尾黄素能显著抑制肝星状细胞中Ⅰ型胶原及活化标志α-SMA mRNA的表达,增强肝星状细胞中MMP-13 mRNA的表达,降低TIMP-1 mRNA的表达。结论:鸢尾黄素既可以有效地抑制肝星状细胞的活化,同时又能减少肝星状细胞胞外基质的合成以及增加胞外基质的降解,因而具有潜在的抗肝纤维化的作用。

Effects of tectorigenin on the secretion of extracellular matrix in Hepatic stellate cells

AIM: To investigate the effect of tectorigenin on the secretion of extracellular matrix(ECM) in Hepatic stellate cells.METHODS: HSC-T6 cells were incubated with tectorigenin at different concentrations,the mRNA expression of α-SMA,pro-collagen-α1(Ⅰ),MMP-13 and TIMP-1 were detected by real-time PCR.RESULTS:Tectorigenin significantly decreased the expression of α-SMA in HSC-T6,as well as the mRNA expression of pro-collagen-α1,and tectorigenin significantly elevated the mRNA expression of MMP-13,while decreasing the mRNA expression of TIMP-1.CONCLUSION: Tectorigenin could reduce the synthesis and secretion of type Ⅰ collagen in HSC,thereby reducing the deposition of extracellular matrix;the other hand,tectorigenin could promote the degradation of extracellular matrix,so as to have anti-fibrosis potentials.