鲜壁虎冻干粉诱导C6胶质瘤细胞凋亡的血清药理学研究

目的探讨鲜壁虎冻干粉对C6胶质瘤细胞凋亡的影响。方法将30只小鼠随机分为3组，分别给予顺铂腹腔注射，口服鲜壁虎冻干粉及口服等量蒸馏水。20天后，取小鼠血清体外处理C6胶质瘤细胞。通过形态学分析、流式细胞仪及TUNEL法，观察细胞凋亡情况；S—P免疫组化法检测细胞凋亡相关基因bcl-2、bax的表达水平。结果形态学变化、流式细胞仪分析和TUNEL法结果均证实含鲜壁虎血清在体外可诱导C6胶质瘤细胞凋亡；鲜壁虎组与空白对照组比较，细胞内的bcl-2基因表达无明显变化，bax基因表达升高。结论含鲜壁虎血清能够诱导细胞凋亡，其机制可能与上调bax基因有关。

Study on Sero pharmacology of Fresh Gecko Swinhonis Gunther Freeze dried Powder in Inducing Cell Apoptosis of C6 Glioma Cells in Mice

Objective To explore the effect of Gecko Swinhonis Gunther freeze dried powder (GFP) in inducing apoptosis of C6 glioma cells. Methods Thirty mice were randomly divided into three groups and treated with intraperitoneally injection of cisplatin, orally taken GFP or distilled water respectively. After treatment for 20 days, the serum of mice was collected for treating the C6 glioma cells. The apoptosis state of cells was observed by morphological examination, flow cytometry and TUNEL method, and the cell apoptosis related gene expression of bcl 2 and bax were determined by S P immunocytochemical assay. Results The C6 glioma cell apoptosis induction of GFP contained serum in vitro could be confirmed by morphological examination, flow cytometry analysis and TUNEL method. Comparison between the GFP treated group and the blank control group on intracellular bcl 2 gene expression showed no difference, but bax gene expression was higher in the GFP treated group. Conclusion GFP contained serum could induce C6 glioma cell apoptosis, its mechanism might be related with the up regulation of bax gene.