老年冠心病患者血浆中肿瘤坏死因子及其基因多态性的研究

目的　对老年冠心病患者血浆肿瘤坏死因子 (TNF)水平及其基因多态性进行分析 ,探讨其在冠心病发病中的作用。方法　用ELISA及PCR法测定 40例冠心病患者及 30例健康对照者血浆TNF水平及TNF等位基因频率。结果　TNF等位基因频率分布 ,两组之间比较差异无显著性意义 (P >0 .0 5 ) ,而血浆中TNF水平 ,冠心病组明显高于对照组 ,两组之间比较差异有显著性意义 (P <0 .0 0 1) 。结论　TNF基因多态性在冠心病发病机理中可能不起重要作用。TNFA或TNFB基因多态性与冠心病患者血浆TNF水平升高没有关系。

Study of tumor necrosis factor and its gene polymorphism in old patients with coronary heart disease

Objective To analyse the tumor necrosis factor (TNF) and its gene polymorphism in old patients with coronary heart disease (CHD) and its clinical significance.Methods The plasma levels of TNF and its gene polymorphism in 40 patients with CHD were measured by polymerase chain reaction (PCR) and sandwich enzyme linked immunosorbent assay (ELISA) and 30 healthy subjects were used as the control.Results The frequency of TNF gene polymorphism in CHD group was not different significantly from that in the control group,but the level of plasma TNF in CHD patients was markedly higher than that in control.Conclusions TNF gene polymorphism may be not a correlative gene of essential CHD.Neither TNFA nor TNFB gene polymorphism is related to the elevation of plasma TNF a level.Thus,other factor may be more important in CHD.