移植肾功能延迟恢复患者肾小管上皮细胞凋亡及Bcl-2和Bax蛋白的表达

目的:探讨移植肾功能延迟恢复(DGF)患者肾小管上皮细胞凋亡率与凋亡相关基因bcl-2和Bax表达水平的关系,阐明DGF病理损伤机制。方法:586例同种异体肾移植后63例发生DGF患者作为DGF组,观察分为DGF期和恢复期2组,对照组为10例移植术后肾功能正常的志愿入组者。行肾组织穿刺活检病理切片进行原位末端标记检测细胞凋亡率,采用免疫组织化学法检测肾组织凋亡相关基因Bcl-2和Bax表达水平。结果:DGF组患者肾小管细胞凋亡率明显高于对照组(P<0.01);与对照组比较,DGF组患者肾组织Bcl-2蛋白表达水平降低(P<0.05),而Bax蛋白表达水平增高(P<0.001),Bcl-2/Bax比值降低,DGF期患者肾小管细胞凋亡率与Bcl-2蛋白表达水平呈负相关关系(r=-0.52,P<0.05),与凋亡促进基因Bax表达水平呈正相关关系(r=0.63,P<0.05),DGF恢复期患者肾小管上皮细胞凋亡率与Bcl-2和Bax表达无相关性。结论:DGF患者移植肾小管上皮细胞凋亡率增高,与凋亡相关基因蛋白表达水平有关,提示肾小管上皮细胞凋亡可能参与了DGF的病理损伤过程。

Apoptosis of tubular epithelial cells and expressions of Bcl-2 and Bax proteins in delayed graft function patients

Objective To explore the relationship between the apoptotic rate of tubular epithelial cells and the protein expression levels of Bcl-2 and Bax proteins in delayed graft function(DGF) and discussed its correlation with pathogenic mechanism of DGF.Methods 63 patients suffered from DGF in 586 patients who received the allograft renal transplantation were used as DGF group,the observation values were detected in DGF and recovery periods.10 cases of normal renal function after transplantation were used as contr...