| 大鼠尿中2-苯甲酰肼叉-1,3-二噻茂烷及其代谢产物定量研究 |
| |
| 引用本文： | 王宇,李正化.大鼠尿中2-苯甲酰肼叉-1,3-二噻茂烷及其代谢产物定量研究[J].中国药理学与毒理学杂志,1993,7(3):218-221. |
| |
| 作者姓名： | 王宇 李正化 |
| |
| 作者单位： | 华西医科大学药学院新药设计研究中心 华西医科大学制药厂，成部 610041，华西医科大学药学院新药设计研究中心，公共卫生学院环境卫生教研室 成都 610041 |
| |
| 基金项目： | “七·五”国家重点科技攻关项目 |
| |
| 摘 要： | 本文建立了线性梯度洗脱,两波长切换检测和两内标法测定染毒大鼠尿中2苯甲酰肼叉-1.3二噻茂烷(BADYH)及其代谢产物的反相高效液相色谱方法.研究了大鼠ig不同剂量BADYH后,经尿排泄的DADYH及其代谢产物累积量-时间过程.结果表明,BADYH及其代谢产物累积排泄量占染毒总量的39.7％。其中主要代谢产物丙酮缩肝叉1.3二噻茂烷,占23.6％;肼叉1.2二噻茂炼占14.0％
|
| 关 键 词： | 2苯甲酰肼叉 1 3二噻茂烷 代谢产物 高效液相色谱 |
Determination of benzoic acid-1,3-dithiolan-2-ylidenehydrazide and its metabolites in rat urine |
| |
| WANG Yu,LI Zheng-Hua,GAO Ning.Determination of benzoic acid-1,3-dithiolan-2-ylidenehydrazide and its metabolites in rat urine[J].Chinese Journal of Pharmacology and Toxicology,1993,7(3):218-221. |
| |
| Authors: | WANG Yu LI Zheng-Hua GAO Ning |
| |
| Abstract: | A simple,reliable reverse phase-high performance liquid chromatography method with linear gradient elution in two wave lengths and two internal standards was discribed for the simultaneous determination of benzoic acid-1,3-dithiolan-2-ylidenehydrazide(BADYH,Ⅳ)and its three principal metabolites: 1,3-dithiolan-2-ylidenehydrazine(Ⅰ),acetic acid-1,3-dithiolan-2-ylidenehydrazide(Ⅱ)and pyruvic acid-1,3-dithiolan-2-ylidenehydrazone(Ⅲ)in rat urine.The mean recoveries were more than 91%,the coefficients of variation were less than2.46%.BADYH and its metabolites have been determined in rat urine after 3 doses of BADYH.The experimental results show that the main metabolites excreted in rat urine are(Ⅲ)accounting for 23.6%,and(Ⅰ)accounting for 14.0%.The toxic mechanism of BADYH is also discussed. |
| |
| Keywords: | Benzole acid-1 3-dithiolan-2-ylidenehydrazide Metabolites High performance liquid chromatography |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
