黄柏饮片光谱成像指纹图谱的研究

目的建立黄柏饮片的光谱成像指纹图谱,为其质量控制提供新的方法。方法应用电可控液晶滤光光谱成像装置,测定10种不同市售来源的黄柏饮片,光谱分辨率为5nm,光谱覆盖范围为480～680nm,空间分辨率为4000×4000。从成像光谱立方体中提取特征光谱,构建其指纹图谱;采用标准欧氏距离等聚类分析方法解析其指纹图谱。结果黄柏饮片的光谱成像指纹图谱用于其品种和质量的鉴定,结果与性状,显微及理化鉴定结果相吻合。结论光谱成像分析技术可用于中药指纹图谱的构建和质量评价,操作方法简便、快速、无损。

Spectral imaging fingerprint of Cortex Phellodendri Chinensis pieces

Objective The spectral imaging method was used to establish the fingerprint for the pieces of Cortex Phellodendri Chinensis, which was a potential method and would be applied to quality control of Chinese materia medica (CMM). Methods Ten samples came from different drug stores were tested by the liquid crystal tunable filter apparatus. The spectral resolution was 5 nm, the spectral range was from 480 to 680 nm, and the spatial resolution was 4 000 × 4 000. The characteristic spectra obtained from the imaging spectrometer cubes were used to construct the fingerprint of Cortex Phellodendri Chinensis ; the cluster analysis methods, such as standard Euclidean distance, were used to analyze the fingerprint. Results The identification results by the spectral imaging fingerprint accorded well with the result by the traditional biology and chemistry analysis method. Conclusion The spectral imaging analysis method can be used to construct the fingerprint and evaluate the quality of CMM. The testing course is convenient, quick, and noninvasive.