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| 三甲基硅烷衍生化GC-MS研究延胡索中 水溶性非生物碱类化学成分 | |
| 引用本文： | 刘振华,王如伟,何厚洪,姜迅知,叶剑锋,曾苏,胡季强.三甲基硅烷衍生化GC-MS研究延胡索中 水溶性非生物碱类化学成分[J].中国中药杂志,2012,37(14):2108-2112. |
| 作者姓名： | 刘振华 王如伟 何厚洪 姜迅知 叶剑锋 曾苏 胡季强 |
| 作者单位： | 1. 浙江大学药学院,浙江杭州310058;浙江康恩贝制药股份有限公司,浙江杭州310052 2. 浙江康恩贝制药股份有限公司,浙江杭州310052;浙江中医药大学,浙江杭州310053 3. 浙江康恩贝制药股份有限公司,浙江杭州,310052 4. 浙江大学药学院,浙江杭州,310058 |
| 基金项目： | 浙江省重大科技专项(2004C13022) |
| 摘 要： | 目的:研究延胡索Corydalis yanhusuo中水溶性非生物碱类化合物。方法:延胡索的80%乙醇提取物经DA201大孔树脂柱吸附,收集水洗部位,该水洗脱部位再经732阳离子树脂交换,其水洗脱组分干燥后进行三甲基硅烷衍生化。采用GC-MS检测分析,NIST 2005标准谱库结合具体衍生物的MS/MS质谱图进行化合物结构推断。结果:GC-MS共检出约50个色谱峰,初步鉴定了其中的16个化合物,均为含有羟基或羧基的大极性化合物。结论:16个化合物为首次从延胡索中发现,该研究结果为延胡索的深入开发提供了科学依据。 |
| 关 键 词： | 延胡索 水溶性非生物碱类化学成分 三甲基硅烷衍生化 GC-MS |
| 收稿时间： | 9/7/2011 12:00:00 AM |
Water-soluble non-alkaloid chemical constituents contained in Corydalis yanhusuo by trimethylsilyl derivatization GC-MS |
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| LIU Zhenhu,WANG Ruwei,HE Houhong,JIANG Xunzhi,YE Jianfeng,ZENG Su and HU Jiqiang.Water-soluble non-alkaloid chemical constituents contained in Corydalis yanhusuo by trimethylsilyl derivatization GC-MS[J].China Journal of Chinese Materia Medica,2012,37(14):2108-2112. | |
| Authors: | LIU Zhenhu WANG Ruwei HE Houhong JIANG Xunzhi YE Jianfeng ZENG Su and HU Jiqiang |
| Institution: | College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou 310052, China;Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou 310052, China;College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China;Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou 310052, China;Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou 310052, China;Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou 310052, China;College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;Zhejiang Conba Pharmaceutical Co., Ltd., Hangzhou 310052, China |
| Abstract: | Objective: To study the water-soluble non-alkaloid chemical constituents of Corydalis yanhusuo. Method: The 80% alcohol extracts of C. yanhusuo passed through DA201 macroporous resin. Eluted fractions were collected and passed though 732# cation exchange resin. Water eluate was collected, dried and derived with trimethylsilane. Gas Chromatography/Mass Spectrometry and NIST 2005 library were adopted for MS/MS mass spectrogram to infer the compound structure. Result: Sixteen compounds were tentatively identified from about fifty peaks detected by GC-MS and identified as hydroxyl and carboxyl polar compounds. Conclusion: These sixteen compounds were found for the first time in C. yanhusuo. The results provide scientific basis for in-depth development of C. yanhusuo. |
| Keywords: | Corydalis yanhusuo water-soluble non-alkaloid chemical constituent trimethylsilyl derivatization GC-MS |
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