AFMC选择性增强TRAIL对肺癌A549细胞毒性

目的:研究5-烯丙基-7-二氟甲氧基白杨素(AFMC)选择性增强肿瘤坏死因子凋亡诱导配体(TRAIL)对人肺癌A549细胞毒性作用.方法:体外培养人肺癌A549细胞和人胚肺WI-38细胞.全自动生化分析仪测定细胞上清液乳酸脱氢酶活性.碘化丙啶(pI)染色流式细胞术(FCM)分析细胞凋亡率.结果:AFMC与TRAIL合用...

5-Allyl-7-gen-difluoromethoxychrysin selectively enhances cytotoxicity of human lung cancer A549 cells to TRAIL

Objective To investigate whether 5-Allyl-7-gen-difluoromethoxychrysin(AFMC) selectively enhances the cytotoxic effect of human lung cancer A549 cells to TNF-related apoptosis-inducing ligand(TRAIL).Methods Human lung cancer A549 cell line and human embryonic lung WI-38 cell line were cultured in vitro.The lactate dehydrogenase activity was evaluated by automatic biochemisitry analyzer.The apoptotic rate was examined by flow cytometry using PI fluorescence staining.Results AFMC synergetic act with TRAIL increased the cytotoxicity in human lung cancer A549 cells by assay based on a LDH release.The CI value for combination of AFMC and TRAIL was 0.019.In embryonic lung WI-38 cells by co-treatment with AFMC and TRAIL at suboptimal concentration had no apparente cytotoxicity.Flow cytometry analysis with PI staining indicated that the apoptotic rate in human lung cancer A549 cells by co-treatment with AFMC and TRAIL at suboptimal concentration for 24 h was 37.80% ± 1.78%.The apoptotic rate in human embryonic lung WI-38 cells at the same condition was 0.35% ± 0.12%.Conclusion AFMC at subtoxic concentration possess augmentation of TRAIL induced cytotoxicity in human lung cancer A549 cells.