热矿泥浴对大鼠佐剂性关节炎的作用及机制

目的观察热矿泥浴对大鼠佐剂性关节炎(AIA)的治疗效果及作用机制. 方法 Wistar大鼠按 AIA造模后,随机分为 3组,分别给予热矿泥浴、普通黄泥浴共19 d,空白对照组则不予处理.连续观察关节炎症积分,第 30天取标本,检测血清肿瘤坏死因子(TNF α)含量,关节的组织病理学改变,原位杂交法观察基质金属蛋白酶-1(MMP-1)和细胞间黏附分子( ICAM-1) mRNA在关节滑膜和软骨中的表达. 结果热矿泥浴能显著降低AIA的关节炎症程度,包括炎症积分,以及组织切片的滑膜细胞增生和软骨侵袭.热矿泥浴还能显著降低血液中的TNF α含量,抑制滑膜和软骨细胞的 MMP-1 mRNA以及软骨细胞 ICAM-1 mRNA的表达.普通黄泥浴也能抑制软骨细胞的ICAM-1 mRNA的表达. 结论热矿泥浴能显著抑制大鼠 AIA的关节炎症,其机制与抑制TNF α的产生以及 MMP-1和 ICAM-1在滑膜和软骨中的表达有关,且这种作用不单纯是泥浴的物理作用,可能主要是热矿泥浴的化学和生物学作用所致.

Therapeutic effect and mechanism of thermal mineral mud-bath against adjuvant-induced arthritis in rats

AIM:To observe the therapeutic effect and mechanism of thermal mineral mud-bath(TMM) against adjuvant-induced arthritis(AIA) in rats. METHODS:Wistar rats with AIA were divided into three group randomly, treated with TMM or loess mud-bath(LM) for 19 days,respectively.The control group was treated with nothing.The arthritic scores were observed constantly until the 30th day,then the specimen of blood and joints was obtained.The serum tumor necrosis factor α (TNF α )were measured by ELISA, the histopathology of the inflamed ankle were observed,and the expression of matrix metalloproteinase 1(MMP-1) mRNA and intercellular adhesion molecule 1(ICAM-1) mRNA in the synovium and cartilage were detected using in situ hybridization. RESULTS:TMM significantly decreased the severity of arthritis,including arthritic scores,synovium cells proliferation and cartilage erosion in histopathology.Besides,TMM clearly deduced the content of serum TNF α and suppressed the expressions of MMP-1 mRNA in synovium cells and chondrocytes as well as ICAM-1 mRNA in chondrocytes.Meanwhile,LM also suppressed the expression of ICAM-1 mRNA in chondrocytes. CONCLUSION:TMM significantly inhibited adjuvant-induced arthritis in rats, with suppression of serum TNF α production and the MMP-1 and ICAM-1 mRNA expression in synovium and cartilage. This therapeutic effect attributed to the colligation of physical chemical and biological effects from the thermal mineral mud-bath.