维生素A和锌过量摄入对大鼠脾脏的损伤作用

目的探讨维生素A（VA）、锌（Zn）摄入过量对成年大鼠脾脏抗氧化能力和细胞周期的影响。方法通过灌胃造成VA、Zn单独及联合摄入过量的成年Wistar大鼠动物模型，分别在实验第2周末、第3周末处死半数动物，制备脾脏组织匀浆，测定脾脏总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH—Px）、超氧化物歧化酶（SOD）活力及丙二醛（MDA）含量；流式细胞术测定脾脏细胞周期。结果实验第3周末，各实验组脾脏T．AOC水平均低于对照组；联合组、Zn组SOD含量明显高于对照组（P〈0．05）；VA组和联合组S期和G2／M期细胞较对照组明显增多（P〈0．05）。结论短期大剂量补充VA和（或）Zn可阻抑脾脏的抗氧化能力和细胞周期，以联合过量作用更为明显。

Effects of excessive Vitamin A, Zn or both intaken on rat spleen

Objective To study the effect of excessive VitA,Zn or both intaken on lipid peroxidation and antioxidant status and cell cycle of normal adult rat spleen. Methods The rat model was induced by lavaging solution containing VitA,Zn or both.After 2w and 3w,half numbers of every group were killed and measured the contents of total antioxidation pability(T-AOC),the glutathione peroxidase(GSH-Px),the malondialdehyde(MDA),the superoxide dismutase(SOD),and the cell cycle of spleen was detected by flowcytometry. Results The T-AOC decreased significantly in every test group than the control after 3w.The SOD level of the united group and Zn group were significantly higher than the control.The expression in cell cycle blocked in the S and G_2/M phase increased significantly in the VitA group and united group. Conclusion Excessive VitA,Zn or both intaken could lead to a certain extent damage in rat spleen in short term,especially VitA and Zn both intaken.