| HPLC测定复方双花口服液中绿原酸的含量 |
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| 引用本文： | 余杰,匡辽,林凡胜,戴俊东,孙毅坤. HPLC测定复方双花口服液中绿原酸的含量[J]. 中国执业药师, 2010, 7(3): 32-34 |
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| 作者姓名： | 余杰 匡辽 林凡胜 戴俊东 孙毅坤 |
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| 作者单位： | 1. 北京源生素源生物科技有限公司,北京,102212
2. 北京中医药大学中药学院,100102 |
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| 摘 要： | 目的：建立复方双花口服液中绿原酸的含量测定方法。方法：采用高效液相色谱法,色谱柱为迪玛C18（250×4.6mm,5μm）;流动相：0.4%磷酸-乙腈溶液（90：10）;流速：1mL/min,检测波长：327nm;进样量：10μL。结果：绿原酸在浓度150.72～753.60μg/mL范围内呈良好的线性关系（R2=0.9996）,平均回收率为98.87%（RSD=0.76%）。结论：本方法操作简便、准确、重复性好,精密度高,可作为复方双花口服液的质量控制方法。
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| 关 键 词： | 复方双花口服液 绿原酸 高效液相色谱法 |
Determination of Chlorogenic Acid in Fufang Shuanghua Oral Liquid by HPLC |
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| YuJie,Kuang Liaohong,Lin Fansheng,Dai Jundong,Sun Yikun. Determination of Chlorogenic Acid in Fufang Shuanghua Oral Liquid by HPLC[J]. China Licensed Pharmasist, 2010, 7(3): 32-34 |
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| Authors: | YuJie Kuang Liaohong Lin Fansheng Dai Jundong Sun Yikun |
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| Affiliation: | 1 Beijing Yuansheng Suyuan Biolechnology Co.Ltd,Beijing 102212,China;2 Beijing University of Traditional Chinese Medicine,Beijing 100102,China) |
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| Abstract: | Objective：To establish a method for determining the content of Chlorogenic Acid in Fufang Shuanghua Oral Liquid.Methods：HPLC with Dikma C18 （250×4.6 mm,5 μm）column was used.0.4%H3PO4-Acetonitrile （90：10）as the mobile phase,flow rate was 1.0 mL/min and detection wavelength at 327 nm.Results：There was a good linear relationship at a range of 150.72-753.60 μg/mL for Chlorogenic Acid （R2=0.999 6）.The average recovery rate was 98.87% with RSD of 0.76%.Conclusion：The method is simple,fast and accurate.It can be used for quality control of Fufang Shuanghua Oral Liquid. |
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| Keywords: | Fufang Shuanghua Oral Liquid Chlorogenic Acid HPLC |
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