| Hedgehog信号通路在乳腺癌CD44^＋ CD24^-细胞中激活 |
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| 引用本文： | 陶雅军,毛俊,张晴晴,李连宏.Hedgehog信号通路在乳腺癌CD44^＋ CD24^-细胞中激活[J].山东医药,2011,51(16):33-35,118. |
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| 作者姓名： | 陶雅军 毛俊 张晴晴 李连宏 |
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| 作者单位： | 大连医科大学,辽宁大连,116044 |
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| 基金项目： | 国家自然科学基金资助项目，教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 目的了解Hedgehog信号通路在乳腺癌发生发展中的作用。方法采用免疫磁珠法从无血清培养的乳腺癌悬浮细胞中分选CD44＋CD24-细胞和非CD44＋CD24-细胞,分别接种于NOD/SCID鼠乳腺脂肪垫内,观察成瘤情况。采用real-time RT-PCR技术检测Hedgehog信号通路分子SMO和GLI1在CD44＋CD24-细胞和非CD44＋CD24-细胞中的表达情况。结果分选出的CD44＋CD24-细胞约占细胞总数的2.25%,SMO mRNA和GLI1 mRNA在CD44＋CD24-细胞中的表达均高于其在非CD44＋CD24-细胞中的表达（P均〈0.05）。CD44＋CD24-组乳腺癌发生率为100%（3/3）,发生肺转移、淋巴结和肺转移、肝脏和淋巴结转移各1只;非CD44＋CD24-细胞组乳腺癌发生率为50%（2/4）,未见有转移发生。结论在乳腺癌CD44＋CD24-细胞中Hedgehog信号通路分子表达增强,其与乳腺癌的浸润转移关系密切。
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| 关 键 词： | Hedgehog信号通路 乳腺肿瘤 CD44＋CD24-细胞 NOD/SCID鼠 |
Activation of Hedgehog signal pathway in breast cancer CD+44 CD-24 cells |
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| TAO Ya-jun,MAO Jun,ZHANG Qing-qing,LI Lian-hong.Activation of Hedgehog signal pathway in breast cancer CD+44 CD-24 cells[J].Shandong Medical Journal,2011,51(16):33-35,118. |
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| Authors: | TAO Ya-jun MAO Jun ZHANG Qing-qing LI Lian-hong |
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| Institution: | TAO Ya-jun,MAO Jun,ZHANG Qing-qing,LI Lian-hong(Dalian Medical University,Dalian 116044,P.R.China) |
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| Abstract: | Objective To explore the effect of Hedgehog signal pathway in the progress of breast cancer.Methods CD44+CD24- cells and non-CD44+CD24- cells were selected from cell suspension in the culture system of a serum-free medium by magnetic activated cell sorting system(MACS) and injected in NOD/SCID mice,respectively.Real-time RT-PCR was employed to detect the expression of Hedgehog signal molecule SMO and GLI1 mRNA in CD44+CD24- cells and non-CD44+CD24- cells,respectively.Results CD44+CD24- cells accounted for 2... |
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| Keywords: | Hedgehog signal pathway breast neoplasms CD44 CD24- cell NOD/SCID mice |
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