CCM、TPA诱导人卵巢癌细胞株skov_3 mtDNA突变的研究

目的研究姜黄素（CCM）、佛波酯（TPA）对体外培养的人卵巢癌细胞株skov3m tDNA突变的诱导作用。方法提取细胞DNA,进行m tDNA突变的分析,检测各组细胞之间m tDNA的突变率和突变类型。结果 CCM、TPA诱导人卵巢癌细胞株skov3m tDNA突变为多基因、多位点的突变,其突变热点依次位于CoⅡ基因、ND5基因、Cytb基因和ND4基因。突变类型主要为A→T、T→A、G→A、T→C、A→G、C→T,C→T最为常见。结论人卵巢癌细胞株skov3株m tDNA编码区的CoⅡ、Cytb、ND4、ND5是一个高度多态性和突变性的区域,它与卵巢癌的发生、发展有重要关系。

Effects of CCM, TPA on mtDNA mutation in skov3 of ovary cancer cell line

Objective To explore the effects of CCM,TPA on mtDNA mutation in skov3 of ovary cancer cell line.Methods High molecular weight genomic DNA isolation was performed.DNA sequencing was done to examine mtDNA mutation after PCR procedure.Results The mtDNA gene mutation in skov3 of ovary cancer cell line was the highly polymorphic and mutable region.The hot points of mtDNA mutation were located in the CoⅡ,ND5,Cytb and ND4 gene successively.By DNA sequencing,mtDNA mutation was concentrated on the points of A→T,T→A,G→A,T→C,A→G,C→T.The C→T mutation was the hottest.Conclusion The mtDNA genes（CoⅡ,Cytb,ND4,ND5） are mutability and polymorphism,which have some relations with ovary carcinogenesis.