Detection of respiratory viruses and subtype identification of influenza A viruses by GreeneChipResp oligonucleotide microarray |
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Authors: | Quan Phenix-Lan Palacios Gustavo Jabado Omar J Conlan Sean Hirschberg David L Pozo Francisco Jack Philippa J M Cisterna Daniel Renwick Neil Hui Jeffrey Drysdale Andrew Amos-Ritchie Rachel Baumeister Elsa Savy Vilma Lager Kelly M Richt Jürgen A Boyle David B García-Sastre Adolfo Casas Inmaculada Perez-Breña Pilar Briese Thomas Lipkin W Ian |
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Affiliation: | Jerome L. and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 722 West 168th Street, Room 1801, New York, NY 10032, USA. |
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Abstract: | Acute respiratory infections are significant causes of morbidity, mortality, and economic burden worldwide. An accurate, early differential diagnosis may alter individual clinical management as well as facilitate the recognition of outbreaks that have implications for public health. Here we report on the establishment and validation of a comprehensive and sensitive microarray system for detection of respiratory viruses and subtyping of influenza viruses in clinical materials. Implementation of a set of influenza virus enrichment primers facilitated subtyping of influenza A viruses through the differential recognition of hemagglutinins 1 through 16 and neuraminidases 1 through 9. Twenty-one different respiratory virus species were accurately characterized, including a recently identified novel genetic clade of rhinovirus. |
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