Radiosensitivity and sublethal damage repair in human umbilical cord vein endothelial cells |
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| Authors: | T K Hei M J Marchese E J Hall |
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| Affiliation: | 1. Faculty of Economics and Business, KU Leuven, Naamsestraat 69 3000 Leuven, Belgium;2. Faculty of Economics and Business, University of Amsterdam, Roetersstraat 11 1001 NJ Amsterdam, The Netherlands;3. LRisk, Leuven Research Center on Insurance and Financial Risk Analysis, KU Leuven, Naamsestraat 69 3000 Leuven, Belgium;4. LStat, Leuven Statistics Research Center, KU Leuven, Celestijnenlaan 200B 3001 Heverlee, Belgium;1. Epigenetics in Human Health and Disease Program, Baker Heart and Diabetes Institute, 75 Commercial Road, Prahran, VIC 3004, Australia;2. Epigenomic Medicine Laboratory at prospED Training, Carlton, VIC 3053, Australia;3. Department of Clinical Pathology, The University of Melbourne, Parkville, VIC 3010, Australia;4. Department of Microbiology and Immunology, The University of Melbourne, Parkville, VIC 3010, Australia;5. School of Science, STEM College, RMIT University, VIC 3001, Australia;6. Department of Surgery (RMH), The University of Melbourne, Parkville, VIC 3010, Australia;7. Department of Diabetes, Central Clinical School, Monash University, Melbourne, VIC 3004, Australia;8. Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Sha Tin, Hong Kong;9. Hong Kong Institute of Diabetes and Obesity, Prince of Wales Hospital, The Chinese University of Hong Kong, 3/F Lui Che Woo Clinical Sciences Building, 30-32 Ngan Shing Street, Sha Tin, Hong Kong;10. Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Sha Tin, Hong Kong;11. Biomedical Laboratory Science, Department of Technology, Faculty of Health, University College Copenhagen, Copenhagen, Denmark |
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| Abstract: | The assessment of the comparative radiosensitivity of different parenchymal cells is potentially of great importance in clinical radiotherapy. Previous studies from various laboratories using vessel regeneration techniques have demonstrated that endothelial cells are quite radioresistant, having a Do in excess of 250 rads. The present studies were undertaken to investigate the radiosensitivity of human endothelial cells obtained from umbilical cord veins (HEC) using "colony formation" as an endpoint, and to evaluate their ability to repair sublethal radiation damage (SLD). The endothelial origin of the cells in the surviving colonies was demonstrated by their positive staining for factor VIII antigen. The survival curve for HEC has a Do of about 165 rads, with a moderate initial shoulder. Dose fractionation studies demonstrate a moderate capability to repair SLD, comparable to other human cells studied including fibroblasts and other normal diploid cells in vitro. These studies indicate that HEC, being a terminally differentiated cell type with various differentiated markers, may prove useful in radiobiological and radiocarcinogenesis studies. |
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