Wavefront sensorless adaptive optics optical coherence tomography for in vivo retinal imaging in mice |
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| Authors: | Yifan Jian Jing Xu Martin A Gradowski Stefano Bonora Robert J Zawadzki Marinko V Sarunic |
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| Institution: | 1.Engineering Science, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada;2.CNR-Institute of Photonics and Nanotechnology, via Trasea 7, 35131, Padova, Italy;3.Vision Science and Advanced Retinal Imaging Laboratory (VSRI),Department of Ophthalmology & Vision Science, University of California Davis, 4860 Y Street, Ste. 2400, Sacramento, CA 95817, USA;4.UC Davis Eye-Pod, Dept. of Cell Biology and Human Anatomy, University of California Davis,4320 Tupper Hall, Davis, CA, 95616, USA |
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| Abstract: | We present wavefront sensorless adaptive optics (WSAO) Fourier domain optical coherence tomography (FD-OCT) for in vivo small animal retinal imaging. WSAO is attractive especially for mouse retinal imaging because it simplifies optical design and eliminates the need for wavefront sensing, which is difficult in the small animal eye. GPU accelerated processing of the OCT data permitted real-time extraction of image quality metrics (intensity) for arbitrarily selected retinal layers to be optimized. Modal control of a commercially available segmented deformable mirror (IrisAO Inc.) provided rapid convergence using a sequential search algorithm. Image quality improvements with WSAO OCT are presented for both pigmented and albino mouse retinal data, acquired in vivo.OCIS codes: (170.4460) Ophthalmic optics and devices, (110.1080) Active or adaptive optics, (110.4500) Optical coherence tomography |
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