Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8 |
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Authors: | Li-Bing Liu Feng Xie Kai-Kai Chang Ming-Qing Li Yu-Han Meng Xiao-Hui Wang Hui Li Da-Jin Li Jin-Jin Yu |
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Institution: | 1.Department of Obstetrics and Gynecology, The Fourth Affiliated Hospital of Soochow University, WuXi 214062, Jiangsu Province, China;2.Laboratory for Reproductive Immunology, Hospital & Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai 200011, China |
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Abstract: | To explore whether hypoxia and interleukin 8 (IL-8) regulate the viability and apoptosis of cervical carcinomas cells and the possible mechanism. We evaluated the expression of hypoxia inducible factor-1α (HIF-1α), IL-8 and its receptors (CXCR1 and CXCR2) in cervical cancer and cervicitis tissues by immunohistochemistry. Then the effects of hypoxia and IL-8 on the viability and apoptosis of HeLa and SiHa cells were detected by the SRB and apoptosis assays. Here we observed that the expression of HIF-1α, IL-8 and CXCR1 in cervical cancer tissues was significantly higher than that in cervicitis tissues. Hypoxic condition stimulated the secretion of IL-8 and the expression of CXCR1 and CXCR2 on HeLa and SiHa cells. Recombinant human IL-8 enhanced the viability and reduced the apoptosis in HeLa and SiHa cells. HeLa and SiHa cells cultured in 1% oxygen showed the increased viability and apoptosis, and the former effect could be partly reversed by anti-human IL-8 neutralizing antibody. This data suggested that IL-8 secreted by cervical carcinomas cells induced by hypoxia can stimulate the viability of cervical carcinomas cells in an autocrine dependent manner, and contribute to the pathogenesis of cervical cancer. |
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Keywords: | Hypoxia IL-8 cervical carcer cells viabilty apoptosis |
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