Optical axial scanning in confocal microscopy using an electrically tunable lens |
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| Authors: | Joey M Jabbour Bilal H Malik Cory Olsovsky Rodrigo Cuenca Shuna Cheng Javier A Jo Yi-Shing Lisa Cheng John M Wright Kristen C Maitland |
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| Institution: | 1.Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, 3120 TAMU, College Station, Texas 77843, USA;2.Department of Diagnostic Sciences, Texas A&M University Health Science Center—Baylor College of Dentistry, 3302 Gaston Avenue, Dallas, Texas 75246, USA |
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| Abstract: | This paper presents the use and characterization of an electrically focus tunable lens to perform axial scanning in a confocal microscope. Lateral and axial resolution are characterized over a >250 µm axial scan range. Confocal microscopy using optical axial scanning is demonstrated in epithelial tissue and compared to traditional stage scanning. By enabling rapid axial scanning, minimizing motion artifacts, and reducing mechanical complexity, this technique has potential to enhance in vivo three-dimensional imaging in confocal endomicroscopy.OCIS codes: (170.0110) Imaging systems, (170.1790) Confocal microscopy, (170.3880) Medical and biological imaging, (170.3890) Medical optics instrumentation, (170.6900) Three-dimensional microscopy |
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