Gastroprotective Effect of Cochinchina momordica Seed Extract in Nonsteroidal Anti-Inflammatory Drug-Induced Acute Gastric Damage in a Rat Model |
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Authors: | Ji Hwan Lim Joo-Hyun Kim Nayoung Kim Byoung Hwan Lee Pyoung Ju Seo Jung Mook Kang So Young Jo Ji Hyun Park Ryoung Hee Nam Hyun Chang Jin-Won Kwon Dong Ho Lee |
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Affiliation: | *Department of Internal Medicine, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea.;†Life Science R&D Center, SK Chemicals, Suwon, Korea.;‡Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.;§Kyungpook National University College of Pharmacy, Daegu, Korea. |
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Abstract: | Background/AimsThe major compounds of Cochinchina momordica seed extract (SK-MS10) include momordica saponins. We report that the gastroprotective effect of SK-MS10 in an ethanol-induced gastric damage rat model is mediated by suppressing proinflammatory cytokines and downregulating cytosolic phospholipase A2 (cPLA2), 5-lipoxygenase (5-LOX), and the activation of calcitonin gene-related peptide. In this study, we evaluated the gastroprotective effects of SK-MS10 in the nonsteroidal anti-inflammatory drug (NSAID)-induced gastric damage rat model.MethodsThe pretreatment effect of SK-MS10 was evaluated in the NSAID-induced gastric damage rat model using aspirin, indomethacin, and diclofenac in 7-week-old rats. Gastric damage was evaluated based on the gross ulcer index by gastroenterologists, and the damage area (%) was measured using the MetaMorph 7.0 video image analysis system. Myeloperoxidase (MPO) was measured by enzyme-linked immunosorbent assay, and Western blotting was used to analyze the levels of cyclooxygenase (COX)-1, COX-2, cPLA2, and 5-LOX.ResultsAll NSAIDs induced gastric damage based on the gross ulcer index and damage area (p<0.05). Gastric damage was significantly attenuated by SK-MS10 pretreatment compared with NSAID treatment alone (p<0.05). The SK-MS10 pretreatment group exhibited lower MPO levels than the diclofenac group. The expression of cPLA2 and 5-LOX was decreased by SK-MS10 pretreatment in each of the three NSAID treatment groups.ConclusionsSK-MS10 exhibited a gastroprotective effect against NSAID-induced acute gastric damage in rats. However, its protective mechanism may be different across the three types of NSAID-induced gastric damage models in rats. |
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Keywords: | Cochinchina momordica Gastroprotection Anti-inflammatory agents non-steroidal Arachidonate 5-lipoxygenase Phospholipases A2 cytosolic |
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